SX-1是本项目合作单位发明的一种化学杂交剂，有效成分为2-[3-(4-甲氧基-6-甲基-1,3,5-三嗪-2-基-3-甲基脲基磺酰基]苯甲酸甲酯。SX-1已在育种工作中广泛应用，但其作用机理目前仍不了解，迫切需要解决。我们的前期研究结果显示线粒体和呼吸作用很可能参与了SX-1的杀雄机理，因此本项目拟以油菜为研究材料，对线粒体和呼吸作用参与SX-1杀雄的作用机理进行深入研究，内容包括SX-1对线粒体数量的影响、对线粒体结构的影响、对呼吸链的影响、对线粒体氧化磷酸化的影响、对线粒体蛋白组的影响，以及受SX-1影响的、产物定位于线粒体的雄性不育控制基因的克隆,和运用RNAi方法对其功能进行鉴定等。研究结果有助于了解化学杂交剂SX-1的杀雄机理，可促进其在植物育种中得到更广泛、更科学的应用，并有助于研发更多、更优的化学杂交剂。筛选到的雄性不育控制基因有望用来培育雄性不育品种，服务于育种生产实践。

SX-1 is a chemical hybridization agent invented by the co-operated reasearch unit of this project. Its acting substance is 2-[3-(4-methoxy-6-methyl-1,3,5-triazin-2-yl-3-methyl) ureidosulphonyl] methyl benzoate. SX-1 has been widely used in plant breeding practice. However, its male sterilizing mechanisms are still unknown, and should be urgently studied. In our preliminary experiments it was found that mitochondrion and respiration quite probably play roles in the male sterilizing action of this chemical hybridization agent. Therefore, this project is aimed to figure out the detailed roles of mitochondrion and respiration in the male sterilizing action of SX-1 with rapeseed as the experimental material, including its effects on mitochondrial number, mitochondrial structure, mitochondrial respiratory chains, oxidative phosphorylation activity, and its influence on mitochondrial proteome. Male sterility controlling proteins located in mitochondria and sensitive to SX-1 will be identified during the mitochondrial proteome analysis. Based on these proteins new potential male sterility controlling genes will be cloned and their functions will be identified by RNAi method. The research results will provide elucidation about the male sterilizing mechanisms of the chemical hybridization agent SX-1 and make more wide and scientific application possible in plant breeding practice for this chemical hybridization agent, and will help scientists develop more and better chemical hybridization agents. Furthermore, the new male sterility controlling genes can be used to obtain male sterilizing plants, which can be useful for plant breeding practice.