Improved application of tribenuron-methyl as a chemical hybridizing agent with forchlorfenuron for rapeseed hybrid breeding
Improved application of tribenuron-methyl as a chemical hybridizing agent with forchlorfenuron for rapeseed hybrid breeding
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苯磺隆与氯吡脲化学杂交剂在油菜杂交育种中的应用改进
DOI：
10.1007/s10681-017-2045-7
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发表时间：
2017-10
期刊：
影响因子：
1.9
通讯作者：
Wei-Guo Zhao
中科院分区：
文献类型：
--
作者：
Yong-Hong Li;Dian-Rong Li;Wu-Sheng Liang;Jian-Hua Tian;Jian-Chang Li;Hao Wang;Mao-Teng Li;Xu-Peng Guo;Wen-Jie Chen;Zhen-Lan Zhang;Fei Mao;Wei-Guo Zhao
能诱导植物雄性不育的化学物质或药剂被认为是化学杂交剂，在植物杂交育种中非常有用。苯磺隆（TBM）能有效诱导油菜雄性不育。然而，TBM在油菜育种实践中的应用尚未见报道。在此，用含有0.1-0.9 mg/L TBM的CHA（称为CHA-TBM）处理所有22个供试油菜自交系均诱导雄性不育。从2.0- 3.5mm的花蕾期开始，每隔9d用含0.5mg/LTBM的CHA-TBM处理两次，其最高的诱导不育率仅为54-57%，中毒率为36- 39%。这样的不育率和中毒率表明，TBM不是油菜杂交种选育的理想化学杀雄剂。多次筛选试验结果表明，氯吡脲可以缓解TBM对油菜的毒害作用。以含0.6mg/L TBM和2.1mg/L氯吡脲的CHA处理22个供试油菜自交系，在2.0- 3.5mm的FBS下开始，间隔9天，诱导约96-98%的植株雄性不育，仅约2-4%的供试植株中毒。CHA-TBM-FCF处理不影响油菜植株的雌蕊育性，并且对用该CHA产生的油菜杂交种的雄蕊育性没有遗留影响。使用CHA-TBM-FCF成功地产生了一些油菜杂种（例如，秦蓉1号、秦蓉2号、秦优19号、秦优33号、秦杂优4号和秦杂优5号）。利用SSR标记技术，对CHA-TBM-FCF配制的油菜杂交种F1代种子的杂种优势值进行了测定，结果表明，杂种优势值在93-98%之间。结果表明，适宜浓度的TBM和氯吡脲复配的CHA-TBM-FCF是油菜杂交种选育的理想化学杀剂。
Chemicals or agents that can induce plant male sterility are considered chemical hybridizing agents (CHAs) and are quite useful for plant hybrid breeding. Tribenuron-methyl (TBM) can effectively induce male sterility in rapeseed plants. However, applications of TBM in rapeseed breeding practices have not been reported. Here, treatment with the CHA referred to as CHA-TBM, which contained 0.1–0.9 mg/L TBM, induced male sterility in all 22 tested rapeseed inbred lines. However, the highest induced male sterile rates in the tested rapeseed lines were only approximately 54–57% together with poisoned rates of approximately 36–39%, which were resulted from two treatments of CHA-TBM containing 0.5 mg/L TBM at an interval of 9 days beginning at the floral bud stage (FBS) of 2.0–3.5 mm. Such male sterile rates and poisoned rates caused by CHA-TBM indicated that TBM is not an ideal CHA for rapeseed hybrid breeding. The results of many screening experiments showed that forchlorfenuron can alleviate the toxic effects of TBM on rapeseed plants. Two treatments with the CHA referred to as CHA-TBM-FCF, which contained 0.6 mg/L TBM and 2.1 mg/L forchlorfenuron, at an interval of 9 days beginning at the FBS of 2.0–3.5 mm induced male sterility in approximately 96–98% of plants of all the 22 tested rapeseed inbred lines and poisoned only approximately 2–4% of the tested plants. The CHA-TBM-FCF treatment did not affect pistil fertility of the rapeseed plants, and had no carry-over influence on stamen fertility of the rapeseed hybrids produced with this CHA. Some rapeseed hybrids were successfully produced using CHA-TBM-FCF (e.g., Qinrong 1, Qinrong 2, Qinyou 19, Qinyou 33, Qinzayou 4 and Qinzayou 5). The hybridity values of the F1 seeds of the rapeseed hybrids produced using CHA-TBM-FCF were determined to be approximately 93–98% with the simple sequence repeat (SSR) marker techniques. Together, the results indicate that CHA-TBM-FCF containing TBM and forchlorfenuron at suitable concentrations is an ideal CHA for rapeseed hybrid breeding.
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影响因子：
5.1
作者：
Das, Malay;Reichman, Jay R.;Haberer, Georg;Welzl, Gerhard;Aceituno, Felipe F.;Mader, Michael T.;Watrud, Lidia S.;Pfleeger, Thomas G.;Gutierrez, Rodrigo A.;Schaeffner, Anton R.;Olszyk, David M.
通讯作者：
Olszyk, David M.
影响因子：
2
作者：
Xuan Yao;Yaqin Wang;Xiao-Li Yue;Meng Liu;Kede Liu
通讯作者：
Xuan Yao;Yaqin Wang;Xiao-Li Yue;Meng Liu;Kede Liu
DOI：
10.1073/pnas.0508701103
发表时间：
2006-01-17
影响因子：
11.1
作者：
McCourt, JA;Pang, SS;Duggleby, RG
通讯作者：
Duggleby, RG
影响因子：
4.1
作者：
Pasquer, Frederique;Ochsner, Urs;Keller, Beat
通讯作者：
Keller, Beat
影响因子：
1.4
作者：
A. Aliverdi;M. H. Mohassel;E. Zand;M. Mahallati
通讯作者：
A. Aliverdi;M. H. Mohassel;E. Zand;M. Mahallati