骨髓提取物Buffy coat的软骨修复机制研究

软骨损伤是膝关节常见疾病，又是骨性关节炎的主要病因。目前临床用于治疗关节软骨损伤的方法和药物均难以达到满意的效果。间充质干细胞（MSC）作为软骨组织工程中的一种新型种子细胞，在软骨组织修复上有一定的疗效。MSC在各种因子的刺激下转化为软骨细胞，并生成软骨，但新生的组织与正常软骨组织在生物学特性上存在一定的差异。本课题的前期研究中也发现，自体骨髓提取物Buffy coat对软骨损伤有很好的治疗效果,其主要机制为Buffy coat通过丰富的骨髓源MSC、细胞因子和生长因子等促进组织再生，但其具体机制尚不清楚。因此，本课题利用分子生物学、免疫组织化学、细胞生物学及药理学等方法和手段，分析Buffy coat中能够影响MSC软骨分化及其再生的各种因素，采用其激活剂和阻断剂探讨并阐明骨髓提取物Buffy coat的软骨修复机制，为临床利用Buffy coat修复软骨提供重要的理论和实验依据。

Cartilage injury is a common knee joint disease, but also a major cause of osteoarthritis. The current clinical therapy for articular cartilage injury is hard to achieve satisfactory results. Mesenchymal stem cells (MSC) as a fresh cell source in cartilage tissue engineering, has been shown to play an important roles in cartilage repair. MSC has been transformed to chondrocytes under various stimuli and to produce cartilage tissue and which was showed different properties compared with normal cartilage. In previous study, we have observed that autologous bone marrow derived Buffy coat can significantly enhance repair of cartilage defect. However the mechanisms by which Buffy coat induced cartilage repairmen is not well known. The present project is to investigate effect and it is mechanisms of Buffy coat on cartilage repair and to provide important theoretical and experimental data for its clinical use.