Comparison of Chondrogenic Ability between Mesenchymal Stem Cells and Buffy Coat in Vitro
Comparison of Chondrogenic Ability between Mesenchymal Stem Cells and Buffy Coat in Vitro
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间充质干细胞与血沉棕黄层的体外成软骨能力比较
DOI：
10.4236/jbm.2018.63006
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Zhehu Jin
中科院分区：
文献类型：
--
作者：
Longhao Jin;Zhongye Zhang;Yanqun Liu;Kumar Gurung;Yongjun Jin;Yuanming He;Yu Jin;Zhehu Jin
目的：比较骨髓间充质干细胞（MSCs）和血沉棕黄层细胞体外向软骨细胞分化的能力。方法：取新西兰白色兔骨髓间充质干细胞，体外培养，贴壁传代。将细胞接种在聚乙醇酸（PGA）支架上。（直径3mm，高度2mm）液体培养21天。在相同条件下，不进行传代培养，直接将血沉棕黄层接种到细胞支架中。通过番红-O染色、组织学评分和生化功能检测比较成软骨分化程度。结果：血沉棕黄层组的成软骨分化能力优于MSC组。血沉棕黄层组、MSC组的番红O染色较强。血沉棕黄层组的伯尔尼评分也高于MSC组，并且血沉棕黄层组的硫酸化糖胺聚糖（GAG）总量高于MSC组。结论：血沉棕黄层的成软骨分化能力高于MSC.结果表明，血沉棕黄层在无刺激生长因子的情况下也能分化为亚软骨。因此，血沉棕黄层可作为人工软骨工程的重要来源。
Objective: In vitro comparison of chondrogenic differentiation ability of bone.marrow extracted mesenchymal stem cells (MSCs) and buffy coat. Methods:.MSCs of New Zealand white rabbits were cultured in vitro and adherent cells.were passaged. The cells were inoculated on polyglycolic acid (PGA) scaffold.(3 mm in diameter and 2 mm in height) liquid for 21 days. Under the same.conditions, without subculture, buffy coat was directly inoculated into the cell.scaffold. The degree of chondrogenic differentiation was compared by Safranin-O.staining, histological scoring and biochemical functional detection. Results: The.chondrogenic differentiation ability of the buffy coat group was better than.that of the MSC group. Safranin-O stain was stronger in buffy coat group than.in MSC group. The Bern Score was also higher in the buffy coat group than in.the MSC group and the total amount of sulfated glycosaminoglycans (GAGs).in buffy coat group was higher than that in MSC group. Conclusion: The.chondrogenic differentiation ability of buffy coat is higher than that of MSC..Through this result, it was found buffy coat can also differentiate into.sub-cartilage without stimulating growth factor. Thus, buffy coat can make a.great source in artificial cartilage engineering.
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影响因子：
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作者：
Jin, Cheng Zhe;Park, So Ra;Min, Byoung-Hyun
通讯作者：
Min, Byoung-Hyun
DOI：
10.1007/978-1-60761-999-4_3
发表时间：
2011-01-01
期刊：
MESENCHYMAL STEM CELL ASSAYS AND APPLICATIONS
影响因子：
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作者：
Horn, Patrick;Bork, Simone;Wagner, Wolfgang
通讯作者：
Wagner, Wolfgang
影响因子：
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作者：
Grogan, Shawn Patrick;Barbero, Andrea;Mainil-Varlet, Pierre
通讯作者：
Mainil-Varlet, Pierre
DOI：
10.1073/pnas.95.7.3908
发表时间：
1998-03-31
影响因子：
11.1
作者：
Azizi, SA;Stokes, D;Prockop, DJ
通讯作者：
Prockop, DJ
DOI：
10.5772/8592
发表时间：
2010-03
期刊：
--
影响因子：
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作者：
Zhi-yong Zhang;S. Teoh;M. Choolani;J. Chan
通讯作者：
Zhi-yong Zhang;S. Teoh;M. Choolani;J. Chan