RF影响小鼠睾酮分泌的时间毒性及CaMKI/RORα通路在其过程中的作用

射频场(radiofrequenc fields, RF)辐射是一种重要的环境电磁污染物（EEPs），因无线通讯技术的快速发展,特别是全世界手机用户的急剧增加而备受关注。由于射频场暴露可能导致雄性激素睾酮分泌功能的损害，明确其作用途径和损伤机制迫在眉睫。已知哺乳动物的生理和行为的功能存在昼夜节律，机体在24小时中不同时相对毒物及射频场的敏感性不同。本项目采用时间生物学和生殖内分泌毒理学相结合的策略，研究射频场辐射对睾酮合成的昼夜时间毒性及其特征。通过RNAi和RTFQ-PCR实验等方法确定与射频场暴露昼夜时间毒性相关的生殖系统钟基因Bmal1及其下游睾酮合成调节蛋白StAR，并通过离体Ledig细胞研究CaMKI/RORα通路在其过程中的作用，从而阐明射频场暴露对睾酮合成功能昼夜时间毒性的产生机制。本研究将为射频场暴露引起睾酮合成功能损害的防治提供新的思路。

Radiofrequency fields (radiofrequency fields, RF) radiation is an important environmental electromagnetic pollutants with the rapid development of wireless communication technology and mobile phone users in the world. It has been reported that RF exposure may cause Testosterone （Testosterone， T）synthesis damage, and it is therefore important to clarify the route and mechanism of the RF damage. Circadian rhythms of the physiological and behavioral functions have been widely demonstrated in mammals, which result in different sensitivities of animals to toxicants and RF at different times of a day. This project is to study the circadian rhythm of T synthesis toxicity induced by RF with the combination of chronobiology and reproductive endocrine toxicology. The clock gene Bmal1 and their downstream testosterone synthesis regulatory protein StAR of rats exposed to RF will be ascertained by the RNAi and RTFQ-PCR test, and to associate the functions of CaMKI/RORα signaling pathway in Ledig cell in vitro with the T synthesis rhythm.The aim of the project is to ellucidate the mechanism underlying the chronotoxicity due to the exposure to RF, and to provide with new ways in prevention and treatment of the male T secretion malfunction caused by RF.