在以往NSFC项目工作基础上，结合前期发现：益气活血调控脑出血（ICH）组织lncRNA-mRNA的共表达转录网络。以此为线索，本项目拟靶向lncRNA-miRNA-mRNA三元转录网络进一步探索益气活血法促进ICH组织修复的机制。内容：以益气活血经典方-补阳还五汤及方中的益气药、活血药为工具，采用转录组学技术，捕获敲入/敲出益气药黄芪干预ICH后组织修复的差异三元转录网络；借助生物信息学技术锚定网络中关键的lncRNA-miRNA-mRNA通路；运用双荧光素报告基因和基因沉默/过表达等技术，结合细胞生物学、分子生物学等技术，明确上述三元转录通路的ceRNA机制，验证其在益气活血法促进ICH组织修复中的作用。本研究可望揭示益气活血法促进ICH组织修复的关键三元转录网络，诠释益气活血法“气血共济”治疗ICH的治法协同规律，为益气活血法治疗ICH研究提供新的证据，为治法协同科学性探索新的思路。

Based on our previous NSFC projects, and our finding that qi-tonifying and stasis-eliminating therapy mediates lncRNA-mRNA co-expression of transcriptional networks in intracerebral hemorrhage (ICH), this program aims to target lncRNA-miRNA-mRNA ternary transcriptional network to explore the tissue repair-promoting mechanism of qi-tonifying and stasis-eliminating therapy following ICH. The qi-tonifying and stasis-eliminating prescription-Buyang Huangwu Decoction (BYHWD), qi-tonifying drugs of BYHWD, and stasis-eliminating drugs of BYHWD are used as tools, transcriptome technology is adopted to seize transcriptional networks in brain tissue repair after the qi-tonifying drug huangqi (knock out/knock in) treatment; pivotal lncRNA-miRNA-mRNA pathways are targeted by Bioinformatics technology; dual luciferase report gene, gene silencing and overexpression combination with cytobiology and molecular biology technology are used to investigate the ceRNA mechanism in the ternary transcriptional network, and to confirm the effect of qi-tonifying and stasis-eliminating therapy on the promotion of ICH tissue repair. This study will reveal the vital ternary transcriptional network which implicated in the ICH tissue repair by qi-tonifying and stasis-eliminating therapy, further explain the therapeutic cooperation rule. Our work will provide novel evidence for ICH treated with qi-tonifying and stasis-eliminating therapy, and explore new ideas for therapeutic cooperation.