牡丹、芍药远缘杂交不亲和研究

远缘杂交育种是目前牡丹、芍药品种改良和育种的主要方法，而远缘杂交不亲和一直是制约其快速发展的主要因素。在其他植物育种中，中外学者探讨了一些不亲和的机理，但关于牡丹、芍药远缘杂交不亲和机理的研究还很欠缺。在课题组前期研究中，明确了其杂交不亲和性主要是由受精前障碍造成，通过对比亲和授粉与不亲和授粉的花粉管荧光观察结果，基本确定杂交障碍发生的时间段。本研究拟在前期工作基础上，进一步从牡丹、芍药远缘杂交授粉后雌蕊中保护酶系统以及内源激素的动态变化、不亲和应答相关的柱头差异蛋白质组学分析方面深入研究，初步揭示牡丹、芍药杂交不亲和机理，为杂交育种提供理论和技术支撑。

Distant hybrid breeding is the main methods of cultivar improvement and breeding in tree peony and herbaceous peony. Cross-incompatibility is an important restriction for breeding rapid development. Mechanism of cross-incompatibility had been researched in other plants. But there were little researches in tree peony and herbaceous peony. In preliminary studies, the pre-fertilization barriers were the main factor to cross-incompatibility. The comparison result for fluorescent microscopic observations of pollen tubes showed the time scales of pre-fertilization barriers. Based on the previous researches, the dynamic changes in protecting enzyme system and endogenous hormones, and the analysis on different protein of stigma in pollen-stigma interaction would be further explored. The mechanism of cross-incompatibility between tree peony and herbaceous peony will be revealed, and provide the theoretical and technical support for hybridized breeding.