内皮细胞功能关键指标的高通量筛查及其在ED早期预警中作用的研究

勃起功能障碍（ED）的发生和发展与内皮细胞功能损害有着密切的关系，而且内皮细胞功能的损害常发生于ED症状出现前，因此监测内皮细胞功能改变有助于提高ED的早期预警。尽管目前通过观察一些指标的变化，可以检测内皮细胞功能的改变，但是这些指标均缺乏特异性和敏感性，无法为ED的早期预警、早期诊断、早期治疗及预后的监测，提供帮助。因此筛查具有特异性和敏感性的、观察内皮细胞功能变化的新指标是当前研究的热点。本项目拟应用iTRAQ蛋白质组学技术，动态筛查内皮细胞功能关键蛋白，以期获得阴茎海绵体内皮细胞功能特异性的新指标，用于提高ED的早期预警。这些目标的实现，对于降低ED的发病率，提高人民健康水平，具有重要的理论价值和现实意义。

The occurrence and development of erectile dysfunction (ED)are closely related to the dysfunction of corpus cavernosum endothelial cells (CCECs), which usually happens before the occurrence of ED symptoms. Therefore, monitoring the functional changes in the endodermis can help to improve ED early warning. At present, although the changes of CCECs function can be detected by observing the changes of some indicators, it can not help the early warning of ED much because of the deficiency of specificity and sensitivity. Therefore, screening for the indicators with specificity and sensitivity that can detect the change of CCECs function is a hot spot in present research area. This program aims to obtain new specific indicator for detecting the change of endothelial function of the corpus cavernosum to improve the early warning of ED, by Isobaric tags for relative and absolute quantitation（iTRAQ）technologies of proteomics to screen the key protein of CCECs function. The realization of this aim would have great practical significance and profound strategic influence on lowering the ED incidence rate, improving the people's health standard, boosting the economic development and building a harmonious society.