Hh信号通路是进化上最为保守的信号通路之一，控制了多细胞动物发育过程中细胞的生长及分化。Hh信号通路的异常调节将会导致包括多种癌症及出生缺陷在内的人类严重疾病。Ci作为转录因子，在Hh信号传递过程中处于最为重要的地位。然而，目前对于Ci调控机制的研究还远远不够。我们在实验中发现控制SUMO化修饰的E2/E3对Hh信号通路存在较为重要的调控作用；同时，我们在实验中确定Ci可以被SUMO化特异性地修饰。鉴于SUMO修饰在蛋白质功能调控中的重要作用，其很可能通过SUMO化修饰Ci参与了Hh信号的传递。本项目拟利用模式生物果蝇在in vitro及in vivo两方面开展Ci SUMO化修饰的作用机理的研究。同时，也将针对SUMO化修饰Ci在雄性生殖过程中的生物学功能进行研究。以期揭示SUMO修饰调控Hh信号传递的作用机理及其生物学功能，为最终理解Hh信号转导通路机制及功能调控提供坚实的理论基础。

As one of evolution conserved pathways in vertebrate and invertebrate, Hh signaling is essential for metazoan development. Its malfunction causes a series of serious human diseases, including congenital disorder and cancer. As the transcriptional factor of Hh signaling, Ci contributes to controlling downstream gene expressions, therefore the development. However, underlying mechanisms remain ill defined. In order to understand Hh signaling and the development of metazoan, investigating the regulation mechanism of Ci is very important, of which the key questions are the stability, localization of Ci and the generation of Ci75. In our study, we found that E2 and E3 of Sumoylation modification are involved in the regulation of Hh signaling transduction. In the experiment, we identified Ci as the direct Sumoylation target in Hh signaling by different methods. Protein Sumoylation modification plays very important in the regulation of protein function, especially for nuclear proteins. According facts above, we hypothesize here that Sumoylation is involved in Hh signaling by directly sumoylating Ci. In this study, we will use Drosophila as a model to investigate the mechanism which Hh signaling is regulated by Ci Sumoylation. We will also figure out the biology function of Ci Sumoylation, especially in testis germline stem cell self-renew and maintenance.