随着生活水平改善，饮食结构的变化，高尿酸伴高脂血症发病率逐年升高。中医认为“过食肥甘、醇酒厚味，易运化失司”是高尿酸伴高脂血症的主要病因，脾失健运、肾亏虚致湿浊阻滞，膏脂内生、排泄不利是主要病机。课题组自主研制的以铁皮石斛为君药的铁皮石斛六妙方具“益胃健脾、补肾利湿”功效。前期药理实验表明铁皮石斛六妙方对模型动物具有降尿酸、调血脂、改善肠肾病变，但其作用机制尚未阐明。本课题进一步采用脂肪乳剂/高脂高嘌呤等拟“过食膏粱肥甘”建立高尿酸伴高脂血症模型，用RT-PCR、WB、免疫组化、Elisa等方法，研究基于肠-肾轴LPS-TLR4/Nrf2信号通路调节尿酸转运及其之间相关性分析。从整体、细胞、蛋白/基因等层面，研究铁皮石斛六妙方对肠道功能多环节影响，阐述其“益胃健脾、补肾利湿”的科学内涵，探讨基于肠-肾轴功能的降尿酸调血脂机制，为铁皮石斛复方中药新药开发奠定药理学基础。

The incidence of high uric acid and hyperlipidemia increased year by year with the improvement of living standard, the change of diet structure. TCM believes that "overeatingfeigan, wine Atsumi, easy loss of transportation" is a major cause of hyperuricemia with hyperlipidemia, meanwhile dys-splenism, kidney deficien resulted in dampness block, fat and grease inflexibility, excretion disadvantageous is the main pathogenesis. Dendrobium officinale Six Miao prescription (DOFLMF) has the effect on tonifying stomach and spleen, tonifying kidney and removing dampness through diuresis. Pharmacological experiments showed that DOFLMF had effect on reducing uric acid, blood lipid, improve liver function and intestinal renal disease in spleen and kidney deficiency type high uric acid with hyperlipidemia, but the mechanism has not been elucidated. This research adopts the fat emulsion / high fat hyperpurine diet to establish the high uric acid and hyperlipidemia model, using methods of RT-PCR, WB, immunohistochemistry and Elisa to study intestinal function through"LPS-TLR4/Nrf2" pathway to regulate the "intestinal-kidney" uric acid transport and its correlation analysis; from the whole, cell、 protein / gene levels to know effects of DOFLMF on multilinks of the intestinal tract the function, formulate the mechanism of descending uric acid and characteristics of protecting liver and kidney based on intestinal function, laying the foundation for the development of new medicine for anti hyperuricemia.