动物的离子通道型谷氨酸受体(iGLuRs)是一类配体门控性阳离子通道，介导了大多数动物中枢神经系统中的兴奋性神经信号转导。近年来的研究结果表明植物中的谷氨酸受体介导的Ca2+跨膜转运参与调控根系的生长发育。但是关于谷氨酸受体及Ca2+参与调控根生长发育的下游因子及其分子调控机制，目前知之甚少。本项目以水稻osglr3.1突变体为研究材料，深入研究OsGLR3.1调控主根伸长的分子机制。我们的前期工作表明，osglr3.1突变体在根尖分生区积累较少的Ca2+和谷胱甘肽，并且胞内的谷胱甘肽水平受钙离子的调控。本项目拟通过ATAC-seq技术结合转录组测序等手段，寻找OsGLR3.1调控根尖分生区氧化还原稳态的关键转录因子，深入研究该关键转录因子对根尖分生区氧化还原稳态的调控机制。最终阐明谷氨酸及其受体参与调控水稻根系发育的分子机制。

Animal ionotropic glutamate receptors (iGluRs) function as Glu-activated ion channels that mediate most excitatory neurotransmission in the brain. Plant GLRs mediated-Ca2+ conduction and transport has an important role in controlling plant root growth. However, the molecular mechanisms and the nature of downstream components of GLRs in root development remain largely unknown. In this project, we focus on the characterization of rice osglr3.1 mutant, which showed a short-root phenotype, and will reveal the molecular mechanism of OsGLR3.1 that regulates the primary root elongation. Our previous work showed that the osglr3.1 mutant accumulated less Ca2+ and glutathione in the rice root apical meristem. Furthermore, the glutathione accumulation was dependent on Ca2+. In this project, we use ATAC-seq and transcriptomic analysis to identify the key regulators and elucidate the regulatory mechanism of the key transcriptional factors on the controlling the redox homeostasis in root apical meristem. This project will provides insight into a significant mechanism underlying the role of OsGLR3.1 in controlling root growth in rice, and this project eventually proved important theoretical basis and research methods to reveal the germplasm innovation and yield and quality control mechanism in crops.