椎间盘病是危害人类和动物健康的重要疾病。电针作为现代针灸的代表技术，对犬椎间盘脱出压迫脊髓引发的瘫痪显示出了良好的理气活血效应。前期研究表明，椎间盘脱出压迫脊髓，引起脊髓微循环血流灌注量下降、血管数量减少，进而表现神经细胞变性、坏死等继发性损伤是导致神经传导障碍和动物瘫痪的关键病理变化，对此电针具有显著的改善作用。电针是如何在短时间内改善脊髓微循环血流灌注量，增加微血管数量，减轻损伤并促进神经的再生和恢复脊髓的功能，从而发挥其理气活血效应的机理需要进一步研究。针灸效应，离不开针刺信号对微循环血流量的调节和对内皮细胞功能的影响。本项目将在研究电针对痿证犬脊髓微血管内皮细胞舒缩因子、生长因子、黏附因子和抗炎因子的影响，揭示其对脊髓微循环作用的分子机制的同时，运用诱发电位技术分析其信号从末梢到脊髓病变部位的传递路径及进而改善微循环血流、恢复脊髓功能的微观机制，为揭示针灸作用的机理积累资料。

Disease of the intervertebral disc is a serious disease that endangers the health of human and animal. As a representative of modern acupuncture technique, electroacupuncture has demonstrated a good effect of regulating Qi and blood circulation on the canine paralysis caused by extruded intervertebral disc oppressing the spinal cord. Previous studies indicated that the oppression on spinal cord by extruded disc resulted in decreased blood perfusion in microcirculation and number of blood vessels, and other secondary damages such as degeneration and necrosis of neuron cells, which caused the dysfunction of nerve conduction and paralysis of animals. However, electroacupuncture had displayed a significant improvement for this disorder. But how the electroacupuncture improve blood perfusion in microcirculation and number of blood vessels then promote nerve regeneration and function recovery of spinal cord in short time need further research. Effects of acupuncture has close relationship with microcirculatory blood flow and endothelial cell. This project will study the effect of electroacupuncture on endothelial vasomotor factors, growth factors, adhesion and anti-inflammatory factors of spinal cord capillary of "Wei Syndrome" dog, reveal the molecular mechanism of electroacupuncture on the microcirculation of the spinal cord function, at the same time, analyze the signal transmission pathway from the periphery to the lesion site of spinal cord using evoked potential technique, and accumulate data for revealing the mechanism of acupuncture action.