产脂微生物从生长到脂质积累存在一个过渡期，工业生产通常采用批量发酵工艺，限制了发酵产脂的生产效率。产脂酵母过渡期的关键调控者是AMPK，然而，产脂丝状真菌过渡期的内在调控机制尚不清楚。本课题组前期解析了丝状真菌卷枝毛霉WJ11基因组，发现卷枝毛霉WJ11存在AMPD、AMPK的多个编码基因，在这个过渡期AMPD关键基因表达上调，而AMPK催化亚基基因表达下调，由此推测AMPD与AMPK共同参与调控卷枝毛霉过渡期脂质代谢过程。本课题将采用基因重组、发酵、定量PCR、Western blot、HPLC等方法，系统研究AMPD和AMPK对卷枝毛霉脂质积累的协同调控作用及其分子机制，探讨AMPD、AMPK在卷枝毛霉生长和脂质积累过程的表达调控机制。本课题的研究将进一步完善产脂丝状真菌脂质积累分子机制，为构建生长期连续产脂丝状真菌工程菌种、实现产脂丝状真菌的连续发酵工艺奠定基础。

As a transition from growth to lipid producing stage occurs in most oleaginous microorganism, batch fermentation process has been used for lipid production with oleaginous microorganisms, this has greatly restricted the productivity of microbial lipids. The regulator of this transition in oleaginous yeast is AMPK, however, the regulatory mechanism of this transition in oleaginous filamentous fungi is still not clear. Previously, our group has sequenced the genome of Mucor circinelloides WJ11, multiple genes have been found for AMPD and AMPK respectively, and our preliminary data showed that the expression level of the key gene coding for AMPD is upregulated while the gene coding for the catalytic subunit of AMPK is downregulated in this transition. It is hypothesized that AMPD and AMPK co-regulates lipid accumulation of the transition in this fungus. In this study, genetic recombination technology, fermentation technology, RT-qPCR and Western blot etc. will be used to systematically investigate the synergistic regulatory roles of AMPD and AMPK in lipid accumulation in Mucor circinelloides. In addition, the expression mechanism of AMPD and AMPK during the growth and lipid accumulation phase will also be studied. This project will further improve the molecular mechanism of lipid accumulation in oleaginous filamentous fungi, and lay the foundations for the construction of continuous lipid-producing engineering strains for filamentous fungi, and continuous fermentation process for microbial lipids.