水稻是重要的粮食作物，对水稻花药发育的研究将为阐明其生殖发育机制及遗传改良提供基础。defective microspore and tapetum1（dmt1）是前期获得的一个稳定遗传的雄性不育突变体，受单隐性核基因控制。dmt1花药较小呈白色，小孢子发育时期绒毡层提前降解、小孢子形状不规则和活性氧异常累积。通过图位克隆的方法已将其定位于1号染色体长臂上129kb区间内，该区间之前未见花药发育相关基因的报道。本课题拟在已有的工作基础上通过精细定位克隆DMT1基因，细胞生物学方法分析突变表型，基因特定表达模式、亚细胞定位、转基因水稻及分子调控网络的研究阐明其在水稻花药发育中的功能及分子作用机制。通过本研究可望在理论上揭示该基因与水稻花药发育之间的关系，在应用上为选育出新的不育系奠定基础和指明方向。

Rice was the most important crops in the world, the research on rice anther development will provide the basis for the elucidation of rice reproductive development mechanism and genetic improvement. defective microspore and tapetum1 (dmt1) was a stable genetic male sterile mutant, which was controlled by a single recessive nuclear gene. dmt1 anther was small and white, exhibiting an obvious defect with predegradation tapetum, irregular microspore and abnormal accumulation ROS during the microspore development stage. Using map-based cloning strategy, the DMT1 gene was mapped to a new unreported region-129kb interval on the long arm of Chromosome 1. Base on these findings, a fine mapping will be simultaneously carried out to clone this target gene; the mutation phenotype will be further investigated using cell biology analysis methods; the analysis of the special expression pattern, subcellular localization, transgenic rice and molecular regulator network will be performed to ascertain DMT1 function and molecular mechanism. Through this research, we expected to reveal the relationship between the gene with rice anther development in the theory, to laid foundation and direction for breeding new sterile in application.