水稻雄性不育基因DA1调控花药发育的分子机理研究

水稻是重要的粮食作物，对水稻雄配子发育的研究将为阐明其生殖发育机制及遗传改良提供基础。defective anther1 (da1)是项目组前期通过辐射诱变获得的一个稳定遗传的雄性不育突变体，受单隐性核基因控制。da1花药较小呈白色，在减数分裂后绒毡层和小孢子出现异常。通过图位克隆的方法已分离出目的基因DA1，序列分析表明该其因编码一个功能未知的NEMP蛋白。本课题拟在已有的工作基础上，通过细胞生物学观察与生理生化方法进一步分析其突变表型，结合基因特定表达模式、亚细胞定位、转基因植株及分子调控网络的研究阐明其功能及分子作用机制。通过本研究可加深对水稻花药发育分子机制和调控网络的理解，在应用上为水稻的遗传改良提供理论依据。

Rice is the most important crops in the world, the research on rice male gamete development will provide the basis for the elucidation of rice reproductive development mechanism and genetic improvement. defective anther1(da1) was a stable genetic male sterile mutant derived from radiation mutation, which was controlled by a single recessive nuclear gene. da1 anther was small and white, exhibiting an obvious defect with abnormal tapetum and microspore development after meiosis stage. Using map-based cloning strategy, the DA1 gene has been mapped and cloned successfully, the sequence analysis showed that DA1 encodes a NEMP protein with unknown function. Base on these findings, the mutation phenotype will be further investigated using cell biological observation and physiological biochemistry methods, and the analysis of the special expression pattern, subcellular localization, transgenic rice,interaction protein and molecular regulator network will be performed to ascertain DA1 function and molecular mechanism. This study can deepen the understanding of the molecular mechanism and regulatory network of rice anther development, and provide theoretical basis for rice genetic improvement in application.