共刺激分子B7家族在细胞免疫介导的抗肿瘤免疫中发挥着重要作用，本课题组一直致力于B7家族分子的研究，前期研究结果显示B7-1/CD40L联合可以增强肿瘤免疫，体内外敲减PD-L1、B7-H3均可抑制淋巴瘤细胞株的增殖、诱导凋亡，还可以增加化疗敏感性。B7-H6是近年发现的B7家族另一重要分子，特异表达于肿瘤细胞表面，与NK细胞表面受体NKp30结合后可激活NK细胞的肿瘤免疫，NK细胞的杀伤功能在细胞免疫治疗中也起了重要作用。本课题拟通过检测B7-H6在恶性淋巴瘤中的表达及患者NK细胞的数量和功能，分析其与临床诊断、分期、疗效和预后的关系；通过基因敲减及制备单克隆抗体在体内外研究B7-H6对B细胞淋巴瘤增殖、凋亡、侵袭的作用及相关蛋白和信号通路。结果可揭示B7-H6及NK细胞在B细胞淋巴瘤发生发展中的作用及其下游分子机制，为B细胞淋巴瘤提供新的肿瘤标记物和潜在治疗靶点。

B7 family molecules play important roles in antitumor immunity mediated by cellular immunity and our research team is engaged in the study on the effects of B7 family in hematological malignances.Our previous studies have shown that B7-1 and CD40L played an immunotherapeutic effect on lymphoma synergistically, knockdown of PD-L1 and B7-H3can inhibit proliferation, induce apoptosisin vitro and in vivo, and it is related to chemotherapy sensitivity. B7-H6 is another important molecule in B7 family which is found in recent years. B7-H6 is specially expressed in tumor cell surface, and NK cell surface. NK cells are activated when B7-H6 binds to natural toxicity receptor NKp30 on the surface of NK cell to kill tumor cells. Cytotoxicity of NK cells plays an important role in cellular immune therapy. This research will explore the relationship betweenexpression of B7-H6 and the function of NK cellsin hematologic malignancies and theirclinical significance. The role of B7-H6 in the proliferation, apoptosis, invasion and metastasis in B cell non-Hodgkin’s lymphomas (B-NHL) and related mechanisms will be studied by knockdown of B7-H6 and preparation of anti-B7-H6 monoclonal antibody in vitro and in vivo. This study is expected to elucidate the role of B7-H6 in tumorgenesis and progression in B-NHL and its downstream molecular mechanisms, provide new tumor biomarkers and potential therapeutic targets for B-NHL.