以植物源活性化合物为先导进行合成和结构修饰是新型绿色杀虫剂创制的重要手段。二萜生物碱是具有杀虫作用的乌头、翠雀属植物的特征性成分。前期从乌头属植物中发现了1个具有显著活性的C19型二萜生物碱—chasmanthinine，初步构效关系研究表明C8和C14位取代基对活性影响重大，作用机制研究显示其杀虫机理可能与胞内钙离子调控相关。由此，本申请拟在前期研究的基础上，以chasmanthinine为先导，采用生物电子等排原理，设计合成两个系列的C19二萜生物碱，用于活性和构效关系研究，以期得到高活性的新型植物源杀虫剂。同时采用电生理技术，从分子水平研究活性成分对钙离子通路的影响，探讨其杀虫作用机制，为高效、低毒、作用机理明确的新型植物源杀虫剂的创制奠定基础。

The synthesis and structure modification of active natural lead compounds is an important approach for the discovery of novel green pesticides. Diterpenoid alkaloids are the characteristic and active constituents of genus Aconitum and Delphinium plants with insecticidal activity. In the previous phytochemical and insecticide activity studies of the genus Aconitum plants, we got a C19-diterpenoid alkaloid, chasmanthinine, with significantly antifeedant activity. The C8 and C14 substituents have significant influence on activity based on the preliminary structure-activity relationship studies. Initial mechanistic investigation revealed that compound may act on calcium ion channel. On the basis of previous research, we plan to design and synthesis two series of C19 diterpenoid alkaloids on the principle of bioisosterism, to discover new botanical insecticides with high activity and simple structure. At the same time, the mechanism studies about Ca2+ signaling will be detailed discussed at cell and molecular levels. The implementation of the project will provide important information for the development of new type botanical insecticide with higher activity, lower toxicity, and clear interaction mechanism.