猪流行性腹泻病毒(PEDV)引起的猪流行性腹泻(PED)给养猪业造成了巨大的经济损失。PEDV的囊膜纤突（Spike, S）是主要的免疫原蛋白，可诱导中和抗体产生并提供免疫保护。目前尚无精确定位PEDV S蛋白B细胞表位的报道。本研究拟表达PEDV S蛋白，制备PEDV单克隆抗体。将纯化的抗体与表达复合外源多肽的噬菌体库进行生物淘选鉴定与该单抗能特异结合的噬菌体。通过DNA提取，PCR和基因测序鉴定PEDV S蛋白B细胞表位。通过免疫荧光实验、定量PCR和病毒中和实验等特性PEDV B细胞表位在抗PEDV感染中的作用机制。本项目将为深入研究PEDV侵染机理研究奠定基础。

Porcine epidemic diarrhea virus (PEDV) may cause porcine epidemic diarrhea, leading to severe economic loss in pig industry. The spike (S) protein of PEDV is an immunogenic antigen, which is responsible for eliciting immune response. At present, no information regarding fine mapping of B cell epitopes in the PEDV S protein is reported. In this study, we will express PEDV S protein, generate its monoclonal antibody (mAb). The purified mAb will be used as target for a biopanning with phage display random peptide library. By DNA extraction, PCR and sequencing, we will identify B cell epitopes in the PEDV S protein. In addition, the function of these epitopes will be characterized by immunofluorescence assays, quantified PCR and virus neutralizing assays. This work will be a basis for investigating invasion mechanisms of PEDV in-depth.