鼻咽癌（NPC）系鼻咽部黏膜上皮细胞发生的恶性肿瘤，易侵袭转移。Jab1和长链非编码RNA（lncRNA）参与了NPC进展，但在侵袭转移中作用不明。我们前期发现：转移NPC组织中Jab1水平明显高于原位肿瘤组织；NPC细胞内Jab1蛋白特异性结合lncRNA ANRIL；Jab1和ANRIL不仅影响细胞的侵袭能力，还影响上皮间质转化（EMT）信号分子表达。因此，我们推测Jab1绑定ANRIL调控NPC侵袭转移。本项目拟开展如下研究：（1）Jab1绑定ANRIL促进NPC侵袭转移的临床意义和生物学功能；（2）Jab1与ANRIL相互作用的分子机理和在调节EMT相关基因和蛋白的表达、功能发挥中的作用。（3）Jab1绑定ANRIL在原位灶-外周血循环肿瘤细胞-转移灶的EMT全程作用。在临床、体外和体内三个层面阐明Jab1绑定ANRIL促进侵袭转移的分子机制，为转移性NPC的靶向治疗提供新策略。

Nasopharyngeal carcinoma (NPC), originating from the epithelial lining of the nasopharynx, is a squamous-cell carcinoma, many patients with NPC will develop distant metastasis, and the prognosis is very poor once metastasis occurs. Jab1 and lncRNA are involved in the progression of NPC, however, the role of Jab1 in invasion and metastasis remains to be clarified. Our preliminary experiment suggested that Jab1 was overexpressed in metastatic NPC tissues compared with non-metastatic tissues; Jab1 binds to lncRNA ANRIL in NPC cells; Jab1 and ANRIL not only affected the invasion and metastasis of NPC but also regulated EMT (Epithelial to Mesenchymal Transition) associated signal molecule. Collectively, we assume that Jab1 regulates invasion and metastasis by interacting with ANRIL in NPC. Our study will be divided into two parts: (1) to illuminate the biological function and clinical significance of Jab1 and ANRIL in invasion and metastasis; (2) to reveal the mechanism by which Jab1 interacts with ANRIL and subsequently regulates EMT associated genes and proteins expression and function. (3) to study the role of Jab1 and ANRIL in the whole course of EMT in primary tumor-circulating tumor cells (CTCs)-metastasis tumor. With experiments on clinical level, cellular level and mouse model, we intend to elucidate the role of Jab1 and ANRIL in regulating the invasion and metastasis of NPC and explore its clinical application, which will provide new targets and intervention strategies for the treatment of metastatic NPC.