铁是细胞赖以生存的必需元素，铁失调会影响细胞正常生理活动，导致机体疾病的发生。铁调素是参与铁代谢并具有抗菌功能的小分子多肽。对铁调素以及铁代谢相关蛋白的研究具有重要的科学意义和应用价值。金钱鱼是一种抗逆能力极强的海水经济鱼类，本研究以金钱鱼为对象，通过组织定位铁调素并检测铁失调时铁调素的表达变化，探索金钱鱼铁调素在体内铁代谢中的作用机制。接着利用金钱鱼肝细胞系，通过添加重组铁调素以及RNA干扰沉默铁调素等技术手段从细胞水平揭示铁调素在鱼类铁代谢中的作用机理。通过抑制性消减杂交文库筛选铁超载相关蛋白以及Hep相关SMAD信号通路中的信号分子，从分子水平探索铁代谢中蛋白的作用机理。此外，通过验证金钱鱼Hep抗菌功能，揭示海水鱼类相关免疫机制。本研究结合器官水平和细胞水平阐明铁调素与铁代谢相关蛋白相互作用机制，增加了海水鱼类铁代谢研究的认识。

Iron is essential for the survival of cell, iron disorder may cause many serious diseases. Antibacterial peptide hepcidin is responsible for iron metabolism.The research of hepcidin and iron-related protein is important and valuable.Spotted scat(Scatophagus argus) is a kind of economic fish with strong resistance. Our study is based on spotted sact. In order to explore the function of hepcidin in iron metabolism in vivo, we identify and characterize hepcidin by the analysis of protein express location and expression patterns. Combine with histochemical and cell biological technology, the function of hepcidin in iron metabolism mechanism is researched after the establishment of hepatocyte cell line, the characterization of hepcidin in iron metabolism is researched in vitro by the addition of recombinant hepcidin to over express hepcidin or RNA interference to knock down hepcidin. The protein related to iron over load and signal molecules in SMAD pathway are expected to receive by suppression subtractive hybridization. The antibacterial activity of spotted scat hepcidin is also studied by the expression of recombinant protein. Research on Spotted scat iron-related protein hepcidin may enhance the understanding of iron metabolism in seawater fish.