纳米零价铁(nZVI)在土壤/地下水有机氯(OCs)污染修复中的应用日益增加，亟需探明nZVI与OCs的复合生物效应。申请人前期研究发现，一定浓度下nZVI和五氯酚(PCP)对线虫的复合效应表现为拮抗，并且nZVI与线虫存在协同去除土壤PCP的潜力。本项目旨在阐明土壤中线虫对nZVI和PCP复合暴露响应机制与影响因素的基础上，构建和优化nZVI-线虫互作体系以协同去除土壤中PCP。将设置单一/复合暴露实验，运用同步辐射、液/气-质联用、转录/代谢组学等技术，系统研究nZVI和PCP在土壤和线虫中的形态转化及对土壤性质的影响，深入剖析线虫基因和代谢水平的响应机制，阐明nZVI性质与浓度、环境条件、生物累积与代谢等因素对nZVI-线虫协同去除PCP的作用机制，明确主控因素，在保障生物安全的基础上提高对PCP的去除效率。研究结果将拓展纳米材料与污染物复合效应研究的方向，为治理土壤污染提供新技术。

With the increasing application of nano-zero valent iron (nZVI) in the remediation of organochlorine (OCs) polluted soils and groundwater, it is urgent to explore the combined biological effect of nZVI and OCs. We have preliminarily found that the combined effect of nZVI and pentachlorophenol (PCP) on nematodes (C. elegans) was antagonistic at certain concentrations; moreover, nZVI and nematodes have the potential to synergistically remove PCP from soil. Based on this preliminary research, this project was aimed to illuminate molecular mechanisms and influencing factors of nematodes responding to the nZVI-PCP co-exposure in soil, and to establish and improve the interplay system of nZVI and nematodes to synergistically remove PCP within the soil. Single- and co-exposure experiments will be conducted, and technologies such as synchrotron radiation, GC/LC-MS, transcriptomics, metabolomics will be applied to reveal: the transformation of nZVI and PCP in soil and nematodes; effects of nZVI-PCP co-exposure on soil properties; and mechanisms of gene and metabolic responses in nematodes upon the exposures. The effect and mechanism of nZVI property and concentration, environmental parameters, and biological factors on the synergistically removal of PCP by nZVI-nematodes will be specifically addressed. By revealing the dominating influencing factors, the removal efficiency of PCP will be improved on the basis of biological security. This project is believed to expand the research direction of the co-effect of NMs and pollutants, and provide new technology for the control of soil pollution.