非编码RNA在表观遗传及转录后调控等层面上调控基因表达并与胚胎发育密切相关。我们基于生物信息学方法预测出潜在与发育关联的长非编码RNA（lncRNA）。利用RNA-seq数据为基础的转录组重建方法并结合功能数据包括组蛋白修饰、cDNA和编码潜能等筛选候选的lncRNA。对候选的发育相关的新lncRNA进行实验验证。采取不同发育阶段的胚胎，通过分子生物学手段确定其时空特异表达模式及转录本。构建候选lncRNA的全长过表达载体及敲除载体，制作候选lncRNA的小鼠模型。通过高通量芯片分析及组织形态学变化，初步确认候选lncRNA的功能，在此基础上，构建lncRNA、miRNA和mRNA的互作网络。本课题的成果将丰富已有lncRNA库，尤其是胚胎发育密切相关的lncRNA，并阐明lncRNA在胚胎发育过程中的作用机制。为今后表观遗传学研究、干细胞分化、发育的调控和肿瘤的风险评估提供新的思路。

Non-coding RNA are closely related to the regulation of gene expression and embryonic development on the level of epigenetic and post-transcriptional. The potential long non-coding RNA (lncRNA) associated with the developmen have been predicted by based on bioinformatics methods. The candidate lncRNAs are screened by using transcriptome reconstruction methods based on RNA-seq data, combined with functional data, including of histone modifications, cDNA, coding potentials screening and so on. The specific expression patterns and transcripts are studied during mouse different development stages. The over-expression vector containing the full length of candidate lncRNA and the knockout vector are constructed except for making mouse models. By high-throughput microarray and histological changes analysis, the functions of the candidate lncRNA are initially identified, initial recognition, furthermore, the interaction network among lncRNA, miRNA and mRNA are constructed. The study of this project will enrich the lncRNA library, especially the lncRNAs which closely related to embryonic development, and clarify the mechanism of lncRNAs during embryonic development. This study will provide new ideas for future epigenetic studies, stem cell differentiation, developmental regulation and tumor risk assessment.