基于紫膜-金属氧化物纳米阵列的食源性致病菌电化学传感新方法研究

食源性致病菌是食品安全的突出问题，本项目拟开展基于紫膜-金属氧化物纳米阵列（PMMONAs）的电化学食源性致病菌传感新方法研究。以紫膜作生物模板，可控地合成具有良好的生物相容性、稳定性、电催化活性的PMMONAs。基于PMMONAs对无毒生物小分子的电催化氧化还原活性和特异性配体对食源性致病菌的识别作用，构建新型电化学细胞传感平台，力争实现对食源性致病菌的无标签、无生物毒性、超灵敏、高选择性、高稳定性、可再生、快速检测，为探索在食品生产、加工、运输、保藏等环节中的食源性致病菌传播、滋生及消除的科学机制提供新技术和新方法，具有重要的科学意义和社会价值，有望获得一系列具有特色的创新性研究成果。

Food-borne pathogen is the prominent problem of food safety. This project aims to fabricate a novel electrochemical cytosensor based on purple membrane-metal oxide nano-arrays (PMMONAs) for the detection of food-borne pathogens. Based on the PM templates, the synthesis strategy of PMMONAs with the excellent biocompatibility, stability and electrocatalytic activity is proposed. Based on the electrocatalytic redox activity of PMMONAs for nontoxic small biomolecules and the specific recognition of ligands for food-borne pathogens, the novel electrochemical cytosensing platform will be established for the label-free, nontoxic, sensitive, selective, stable, renewable and rapid detection of food-borne pathogens. The project will provide new technology and method for exploration of scientific mechanism of infection, propagation and elimination of food-borne pathogens in production, processing, transportation and preservation of food. This project will be of great scientific significance and social value, and innovative research results will be obtained.