藏酋猴 (Macaca thibetana) 是我国特有猕猴动物，也是生物医学中日益广泛使用的模式动物。我们前期的研究表明藏酋猴与其他猕猴相比在许多免疫和糖代谢相关基因上存在较大遗传差异。本研究拟采用二代测序技术对不同发育阶段和性别的藏酋猴血液进行转录组测序，绘制血液转录组图谱，重点分析免疫和糖代谢相关基因，并鉴定不同发育阶段和性别个体间的差异表达基因，探讨年龄和性别对基因表达的影响。鉴定个体间SNPs (单核苷酸多态性位点)，并结合基因组数据，分析SNPs对基因表达的影响，在基因组和转录组水平全面深入的了解藏酋猴免疫和糖代谢相关基因的遗传特征。此外，比较藏酋猴和恒河猴(M. mulatta)、食蟹猴 (M. fascicularis)的血液转录组，在基因表达水平揭示它们在免疫和糖代谢相关基因的差异，以阐明藏酋猴基因表达的特性，为藏酋猴更好的应用于生物医学研究提供必要的理论基础。

Tibetan macaque (Macaca thibetana) is an endemic species to China, and it has been used as animal model in biomedical studies. Previous study found that there were big differences between its immune and glucose metabolic related genes and that of other macaques. In this study, we will apply the next generation sequencing technology to sequence the blood transcriptome of different Tibetan macaques. In order to reveal the genetic background of its immune and glucose metabolic related genes, we will analyze these genes and will explore the influence of age and sex on the gene expression after assembling the blood transcriptomes. Meanwhile, we will identify the SNPs between different individuals, and then test whether the SNPs in the genome and transcriptome of Tibetan macaque will change the expression level of the corresponding genes. Comparative analysis with the previous genomic and the current blood transcriptomic data would provide further insight into the genetic background of the Tibetan macaque. Last, in order to provide theoretical support for developing Tibetan macaque into biomedical model, we will discuss the differences on the immune and glucose metabolic related genes between Tibetan macaque and Chinese rhesus macaque (M. mulatta) and the crab-eating macaque (M. fascicularis) through the comparison of their blood transcriptomes.