血浆miRNA在非小细胞肺癌（NSCLC）诊断及发生发展中的作用已受到关注，但其敏感度和特异性较低。我们前期发现：NSCLC红细胞miRNA表达谱相比健康人群变化显著；其中miR-451a表达丰度最高且差异倍数在7倍以上；NSCLC红细胞对miR-451a有显著富集作用。我们提出红细胞可能会富集NSCLC来源的特异miRNA并参与癌症的发生发展。本课题拟通过miRNA高通量测序、ROC曲线等筛选NSCLC中红细胞差异miRNAs并明确其对NSCLC诊断的敏感度和特异性；通过示踪实验及检测NSCLC不同天龄红细胞中miRNA的表达，明确红细胞通过“NSCLC miRNA→外泌体→红细胞”途径对NSCLC miRNA的富集作用；通过示踪实验、流式细胞术、RT-PCR等，明确红细胞通过“红细胞中NSCLC来源miRNA→红细胞外泌体→NSCLC转移组织”途径对肺癌转移的调节作用及机制。

The role of plasma miRNAs in the diagnosis and development of non-small cell lung cancer (NSCLC) has attracted much attention, but their sensitivity and specificity are low. We found that the expression profiles of miRNAs in NSCLC red blood cells (RBCs) changed significantly compared with healthy people, and the expression abundance of miR-451a was the highest and the difference multiple was more than 7 times, and NSCLC RBCs could enrich miR-451a significantly. We suggest that RBCs may enrich specific miRNAs derived from NSCLC and participate in the occurrence and development of cancer. In this study, we intend to screen differentially expressed miRNAs derived from RBCs in NSCLC and clarify their sensitivity and specificity for the diagnosis of NSCLC by miRNA high-throughput sequencing and ROC curve. Through tracer experiments and detection of the miRNA expression in RBCs of different day ages in NSCLC, we clarify the enrichment effect of erythrocyte on NSCLC miRNAs through the way of "NSCLC miRNA→exosome→erythrocyte". Through tracer experiments, flow cytometry and RT-PCR, we clarify the regulatory effect and mechanism of erythrocyte on metastatic tissues through the pathway of "miRNA from NSCLC in erythrocyte→erythrocyte exosome →NSCLC metastatic tissues".