临床研究表明输注血小板会增加肿瘤患者肿瘤转移及复发风险，肿瘤患者体内血小板微粒水平与肿瘤侵袭性和临床预后显著相关，但血小板在保存过程中血小板微粒释放规律及其对肿瘤细胞生长影响的研究鲜见报道。我们前期研究结果证实，单采血小板随保存时间延长其微粒释放逐渐增多，且单采血小板上清和部分临床用新鲜冰冻血浆对HepG2肝癌细胞增殖具有促进作用。本项目拟在此基础上应用流式细胞、微球捕获、蛋白芯片和基质辅助激光解析飞行时间质谱等技术，研究单采血小板在不同保存期血小板微粒数量和微粒性质的释放规律；应用实时无标记动态细胞分析技术研究不同数量及性质的血小板微粒对HepG2细胞增殖能力的影响；应用生物信息学分析技术预测血小板微粒发挥促进HepG2细胞增殖的信号通路，用分子生物学研究其作用机制。明确单采血小板在保存过程中血小板微粒释放规律和对HepG2细胞的作用及其机制，为降低肿瘤患者血小板输注风险提供实验依据。

The research showed that platelet transfusion increased its metastasis and recurrence rate with cancer patients; A significant correlation between the level of platelet microparticles in patients with tumor invasion and prognosis. Relatively few reported about the rule of platelet particles released and the effects of tumor cell growth. Our preliminary results confirmed that the platelet microparticles released gradually increased with the prolongation of storage time, and apheresis platelets supernatant and parts of fresh frozen plasma used in clinical can promote the proliferation of HepG2 hepatoma cells. Our further study focus on the regularity of platelet microparticles number and properties in different period by flow cytometry, microspheres capture, micRNA identification, protein chip and matrix assisted laser desorption time of flight mass spectrometry techniques; Study on the effect of different platelet microparticles on proliferation of HepG2 cells by real time label free dynamic cell analysis; Study on the possible mechanism of the signal pathway of platelet microparticles level on cancer cells by bioinformatics analysis and molecular biology techniques. It will be clear that the rule of platelet microparticles’ release in the process of platelet storage, the effect on the HepG2 and its mechanism. and provide experimental basis for reducing the risk of platelet transfusion for cancer patients.