重金属镉（Cd）对农田蜘蛛产生各种生理生态的毒性影响。该领域研究虽已取得一定进展，但蜘蛛对镉毒性响应的机理尚不明确。在前期镉污染蜘蛛毒腺转录组测序确定三个编码F-actin差异基因的基础上，本项目围绕F-actin通过Hippo信号通路调控农田蜘蛛毒腺对镉污染的响应机制开展研究：⑴ 克隆并验证蜘蛛毒腺F-actin的三个基因序列；揭示F-actin基因在镉污染蜘蛛不同时期及蜘蛛不同部位的时空表达谱；⑵ 对F-actin基因进行RNA干扰，利用冷冻切片、GC-MS、cDNA全长测序等方法检测RNAi后镉污染蜘蛛毒腺的表型特征；利用ChIP、qRT-PCR等技术，筛选并鉴定Hippo信号通路中与F-actin互作的候选靶标基因，阐释F-actin基因调控蜘蛛毒腺响应镉污染的分子机制。该研究不仅有助于理解镉污染对农田生态系统的毒性影响，而且对镉污染治理和保持农田蜘蛛物种稳定性也具有指导意义。

Cadmium (Cd) can be transferred and accumulated in farmland spider and affect their physiological and ecological traits. Although some progress has been made in this field, the response mechanism of spiders to Cd toxicity is still unclear. Based on the identification of three different genes encoding F-actin by RNA-Seq of spider venom gland under Cd pollution stress, this project focuses on the response molecular mechanism of F-actin to Cd pollution stress in spider venom gland by Hippo signaling pathway: ⑴ to clone and verify the three gene sequences of F-actin in spider venom gland, and to reveal the spatio-temporal expression profiles of F-actin genes in different stages and different parts of spider under Cd pollution stress; ⑵ to study the RNAi of F-actin genes in spider venom gland using injection, to detect the phenotypic characteristics of spider venom gland under Cd pollution stress after RNAi by frozen section, GC-MS, cDNA sequencing; to screen and identify the candidate target genes interactions between F-actin and hippo signaling pathway members by ChIP, qRT-PCR and etc, and to elucidate the molecular mechanism of F-actin gene in regulating spider venom gland response to Cd pollution stress. This study will not only helps to understand the toxic effects of Cd on farmland ecosystem, but also has the guiding significance for Cd pollution control and maintaining the stability of farmland spider species.