随着sRNA在细菌中的调控作用被逐渐发现和重视起来，越来越多的细菌sRNA被预测出来，但极少获得功能验证。在解淀粉芽胞杆菌FZB42中已预测到86个sRNAs，其中73个sRNAs的功能未被注释，尚无调控生防功能的sRNA被鉴定出来。本项目前期我们发现解淀粉芽胞杆菌FZB42的sRNA Bas03参与调控生物膜形成和群集运动，Bas32能够影响产胞效率，直接制约芽胞杆菌生防功能的发挥。通过生物信息学分析和qRT-PCR检测初步确认Bas03和Bas32的作用靶标基因分别为surfactin的合成基因srfAC和产胞基因spoVAE1。本项目将进一步通过lacZ报告基因融合实验、碱基结合位点定点突变和RNA-EMSA手段分析Bas03/Bas32、分子伴侣蛋白Hfq与靶标基因三者之间的互作关系，揭示sRNA和Hfq蛋白的调控机制及生物学功能，为高效稳定的芽胞杆菌生防制剂的研发提供理论基础。

The regulatory mechanism of sRNAs in bacteria has been gradually emphasized. Although many bacterial sRNAs have been predicted, little functional verification has been achieved. In Bacillus amyloliquefaciens FZB42, 86 sRNAs have been verified by differential RNA sequencing and 73 of them have not been annotated. Moreover, no sRNA about biocontrol activity has been identified. Our previous studies demonstrated that sRNA Bas03 could regulate biofilm formation and swarming motility and Bas32 could affect the sporulation efficiency, thus restricting the Bacillus biocontrol activity. Bioinformatic analysis revealed that target genes may be surfactin synthetic gene srfAC for Bas03 and sporulation-related gene spoVAE1 for Bas32. The objective of this study is to further explore the interaction between Bas03/Bas32, chaperone protein Hfq and target genes by lacZ reporter gene fusion, core pair region mutation and RNA-EMSA analysis. The results of this project will shed light on the function and regulatory mechanism of sRNA in Bacillus and provide the theoretical basis for development of highly efficient Bacillus based agents.