急性肺损伤(ALI)是创伤的常见后果和致死性并发症之一。ALI过程中肺表面活性蛋白(SP)-B a-helix的结构、构象及功能有不同程度的改变，其影响着SP功能的维持，在肺损伤过程中有重要的作用。 本项目拟采用膜分子生物学的理论技术方法，研究ALI过程中微生化环境变化对SP-B a-helix在脂质中的构象及功能的影响，明确ALI过程中SP-B a-helix疏水结构域与脂质结构关系变化规律、维持和修复肺泡形态的疏水结构域的分子基础与机制，确定有助于维持和修复ALI肺泡正常功能的SP-B a-helix疏水结构域的结构特征；并以此为基础通过对SP-B a-helix进行分子定向进化设计，使其更有助于肺泡功能的维持和修复。 此项目研究成果有助于深入理解SP-B在ALI过程中的分子机理，并为急性肺损伤治疗提供新的理论依据和手段，为其替补治疗和基于结构类似物的药物设计开发奠定基础。

Surfactant protein B（SP-B） produced by alveolar to lower the surface tension by inserting lipids into the air/liquid interface of the lung is unambiguously necessary for adult pulmonary function. The hydrophobicity of the N-terminal segment assists insertion of the peptide into lipid, the amphipathic helix optimizes the orientation and charge specificity for interaction with specific lipids inbilayer-monolayer ensembles. The relation between SP-B a-helix and lipid in acute lung injury (ALI) was researched by methods of membrane molecular biology, the change of structure and function of SP-B a-helix is helpful to understand the molecular mechanism of ALI, and the maintenance and reparation of surface activity of SP-B was determined. From the informations of orientation, structure and function of SP-B a-helix affected by the environment of ALI, the key hydrophobicity amino acids or structure was analyzed. Through the reseach, determinate evolutionary SP-B mutation which is more suitable to maintain and repair the pulmonary function, can be used for replacement therapy of acute lung injury. It is very important to drug design of analogue and replacement therapy for acute lung injury.