营体内受精的许氏平鮋精子成熟诱导及其获能的生理机制

许氏平鮋（Sebastes schlegelii）与体外受精硬骨鱼类不同，雄鱼精子在雌性卵巢腔中储存并完成受精过程，精子在精巢内成熟存在怎样诱导机制？精子在母体内如何获能并受精？其生理机制尚未揭示。本课题利用转录组学和脂质组学研究技术，测定许氏平鮋生殖交配前后雄鱼转录组学和脂质组学特征，查明精子成熟与排精生理学机制；测定受孕前后雌鱼转录组学和脂质组学特征，查明精子在母体内获能机制。通过对转录组学和代谢组学数据进行统计分析，确定许氏平鮋精子成熟和获能阶段发挥主效作用的候选基因群及血浆性类固醇激素典型标记物种类，进行关键功能基因和类固醇激素合成相关酶和受体蛋白表达研究并分析与脂质代谢通路的关联程度，建立转录组学与脂质组学整合的鱼类生理学研究模型。通过这些研究，在理论上丰富鱼类繁殖生理学研究内容，为揭示鱼类生殖进化策略奠定基础，在实践上为近海岩礁鱼类资源开发与保护性利用提供科学依据。

Different with teleosts in vitro fertilization, sperm of male black rockfish (Sebastes schlegelii) discharged into ovarian cavity and were fertilized there. During this physiology process, what mechanisms regulate sperm maturation and sperm capacitation? However, there is little information regarding this physiology process in ovoviviparous Sebastes schlegeli. To clarify underlying mechanisms of maturation and spermiation, this study focus on physiology traits of transcriptomics and lipidomics before and after mating in males. Furthermore, this study also employ these methods to explore physiology mechanisms underlie sperm capacitation in female ovarian cavity. By integrated analysis of transcriptomics and lipidomics, we could determine candidate gene groups and steroid hormone biomarker during sperm maturation and capacitation, could analyze the correlation between key function genes, steroid hormone synthetase, receptor protein and lipid metabolic pathways, and could establish integrated fish physiology research models of transcriptomics and Lipidomics. These studies could provide an enriched content of fish reproductive physiology, reveal the evolution of reproductive strategy in ovoviviparous fish and display scientific basis for protection and utilization of offshore rockfish.