“瘀痛”不仅是股骨头坏死（ONFH）的重要临床表现，也是病情演化、疗效判断的重要指标和治疗难点。我们前期建立的可视化针刀系统治疗ONFH“瘀痛”疗效满意，但其机制不明。本研究基于疼痛触发点“神经递质-肌纤维代谢障碍”交互作用导致循环障碍是ONFH“瘀痛”病理关键和已有研究基础，提出可视化针刀可能是通过解除疼痛触发点、调控“神经递质-肌纤维代谢障碍”交互作用进而纠正循环紊乱发挥疗效的科学假说。为验证这一假说，本课题拟借助ONFH犬模型，明确疼痛触发点的定位并进行疗效评价；进而针对触发点的电生理改变及痛敏变化、关节组织循环情况、运动功能变化等病理环节，采用电生理学、分子生物学、组织结构学、基因芯片与代谢组学等技术，结合高通量测序靶标筛选、低通量分子验证和特异性阻断剂反证的方法，研究可视化针刀治疗ONFH“瘀痛”的作用途径和分子机制。预期结果将为中医外治法治疗ONFH提供科学依据。

“Stagnate-Pain” is not only an important clinical presentation of osteonecrosis of the femoral head (ONFH), but also an important indicator of disease process and curative effect. Our previous study found that acupotomy visualization therapy could improve the “Stagnate-Pain” of ONFH patients more effectively with eliminating trigger point, but the mechanism remains unclarified. Realizing key links of pathological process based on microcirculation disturbance of trigger point and interaction of “Neurotransmitter-Muscle Fiber Metabolic Disorder”, we suspect that the efficacy of acupotomy visualization therapy may regulate interaction of “Neurotransmitter-Muscle Fiber Metabolic Disorder”, and improve microcirculation disturbance by eliminating trigger point. This subject will firstly locatethe trigger point of ONFH in animal model, then inspect and test correlated index of trigger point, such as muscular tension, microcirculation of joint tissue, hyperalgesia, motor function. Conspiring with technologies and methodologies of electrophysiology, molecular biologtechnology, ultrastructural examination, high-throughput screening combine gene chips and metabolomics, we aim to reveal the mechanisms of acupotomy visualization therapy on “Stagnate-Pain” of ONFH, which may have great value for finding new ways to improve ONFHtreatments and exploit the potential values for Hip-preserving.