紫外线照射可以导致部分植物积累花青素，这是一种自我调节机制。研究发现依光型花青素合成的津田芜菁只受UV-A及UV-B与蓝光复合光诱导。转录组学研究结果表明UV-A特异诱导表达基因群的启动子区中并不含有相同的顺式作用元件，同时部分津田芜菁自交子代个体中存在对UV-A敏感程度不同的现象，这些均表明存在非孟德尔遗传机制的调控。本研究为鉴定UV-A特异诱导芜菁花青素合成过程中可能存在的表观遗传修饰，利用全基因组重亚硫酸盐测序及ChIP-seq的方法对UV-A照射前后津田芜菁全基因组内DNA甲基化修饰程度不同及特异发生组蛋白修饰的位点进行分离。通过生物信息学方法对这些位点与UV-A特异诱导基因群相互比较筛选可能参与该过程的表观遗传学修饰位点，并利用UV-A敏感程度不同的芜菁进行验证。本项目旨在发现UV-A诱导芜菁花青素合成过程中的表观遗传修饰调控规律，为揭示依光型花青素合成分子机制提供新的视角。

UV radiation from the sun can induce anthocyanin accumulation, which is an adaptive adjustment mechanism in plants. Light-dependent anthocyanin biosynthesis in red turnip ‘Tsuda’ was found to be regulated by UV-A (320-380 nm) and co-irradiation of UV-B (280-320 nm) and blue. Our previous study showed that a small set of gene is expressed specifically in response to UV-A, but any common motifs adjacent to these genes could not be found at all, suggesting the presence none-genetic regulation. Recent studies have revealed epigenetic regulation of gene expressions in response to stresses. Epigenetic regulation includes genomic DNA methylation and histone modifications. Although the epigenetic system is becoming to be recognized as a key mechanism for gene expression regulation, few studies have been conducted for light induced stress response. The purpose of this study is to identify putative epigenetic modification involved in UV-A induced anthocyanin biosynthesis in turnip.. Genomic DNA methylation can be analyzed by whole genome bisulfite sequencing (WGBS). Histone modification can be analyzed by ChIP-Seq analysis using modified H3 histone specific antibodies. DNA methylation and histone modifications will be analyzed before and after the exposure to UV-A. Comparing epigenetic modifications and transcriptome profiles based on bioinformatics, the involvement of epigenetic modifications in UV-A specific anthocyanin biosynthesis will be investigated. The results will be verified in different accessions of UV-A sensitive turnip groups.