体内微环境的影响是制约间充质干细胞介导牙周组织再生临床转化的关键环节。本组前期研究发现组蛋白去乙酰化酶抑制剂有促进干细胞分化和调节炎症反应的重要作用，提示组蛋白/非组蛋白调控在炎症微环境下可能具有促进牙周膜干细胞介导牙周组织再生的作用，但功能和机制尚不明确。课题拟研究揭示体内外炎症微环境下组蛋白/非组蛋白乙酰化调控对牙周膜干细胞定向分化、免疫调节和牙周组织再生的作用，通过蛋白印迹、ChIP，IP和质谱分析等实验方法探讨组蛋白/非组蛋白乙酰化调控对牙周膜干细胞参与组织再生的分子机制。有望进一步阐明间充质干细胞参与组织再生关键基因和干细胞命运转归的表观遗传学规律，为牙周炎的治疗提供基础实验和大动物研究证据，以促进间充质干细胞介导牙周再生的临床转化。

The influence of micro-environment on mesenchymal stem cells mediated periodontal tissue regeneration has drawn increasing attention in translational medicine in periodontitis therapy. Our previous study showed that histone deacetylase inhibitors promote differentiation and inhibit inflammatory response of mesenchymal stem cells, which suggest that regulating histone/non-histone acetylation could possess the promise in improvement of periodontal stem cells mediated periodontal tissue regeneration under the inflammatory micro-environment. However, the effect and the underlying mechanisms remain unclear. Herein, the current proposal aims to firstly investigate the effect of histone/non-histone regulation on multiple differentiation, immunomodulation and periodontal tissue regeneration of periodontal stem cells in inflammatory micro-environment in vitro and in vivo. Secondly, to explore the underlying mechanisms by western-blot, ChIP, IP, and mass spectrometry. This proposal may provide a novel principle in elucidating the pivotal molecular mechanism of cell fate decision of odontogenic MSC and mediated tissue regeneration ,therefore may provide scientific and big animal model evidences in future clinical application of periodontal ligament stem cells in periodontal regeneration in inflammatory micro-environment.