角膜移植是治疗角膜盲的唯一手段，由于角膜供体来源匮乏，异种角膜移植是目前研究的热点。猪角膜是理想的异种角膜移植的材料，野生型猪表达α1,3-半乳糖转基因酶（α1,3Gal），而人或非人灵长类动物表达天然抗体，并且针对非Gal抗原的抗体也是异种器官移植的障碍。α1,3Gal基因敲除、转入人补体调节蛋白CD46(GTKO/CD46)猪的成功培育，为猪角膜异种移植提供了希望。本实验通过建立野生型猪、GTKO/CD46猪-猴异种角膜移植动物模型，观察角膜植片存活情况，检测移植术后猴眼局部及全身的细胞因子含量、IgG、IgM、MΦ、CD4+、CD8+T细胞变化、抗α-Gal抗体、膜攻击复合体的含量等免疫学指标，明确GTKO/CD46猪角膜异种移植术后的免疫学特征。通过免疫抑制剂干预，探讨对GTKO/CD46猪-猴异种角膜移植术后植片长期存活的影响，从而为该基因改造猪角膜临床应用奠定理论基础。

Cornea blind can only be treated by allograft corneal transplantation.Since the serious shortage of donated corneas, many studies use pigs as the source animal for corneal xenotransplantation. Natural antibodies directed against α1,3- galactosyl- transferase(α1,3Gal) epitopes of pig organs are a major factor in xenograft rejection after transplantation into nonhuman primates, and antibodies directed against nonGal antigen is another factor.Production of genetically engineered pigs (_1,3-galactosyltransferase gene-knockout [GTKO] and expressing a human complement-regulatory protein [CD46] pigs) make organ xenotransplantation possible.We set up animal model of wildtype ( WT) pig , GTKO/CD46 pig - rhesus monkey corneal xenotransplantation,then rejection signs, cytokine, IgG、IgM、MΦ、CD4+、CD8+T cell, anti-Gal and membrane attack complex of graft, recipients' aqueous fluid and peripheral blood were evaluated. On this basis, recipients are given immunosuppressor , evaluate the survival of the graft to explore the immunological characteristic of GTKO/CD46 pig -rhesus xenotransplantation, which should provide a theoretical basis for clinical corneal xenotransplantation.