微囊藻水华及所产微囊藻毒素（MCs）危及生态安全，已证实产MCs（MC+）与非产MCs（MC-）微囊藻多形成共存竞争互作，关系着水华毒性。前期研究发现木犀草素可抑制MC+微囊藻生长及其MCs合成，表明其修复水华污染的潜质。然而在不同氮磷水平下木犀草素如何调控MC+、MC-微囊藻生长生理和两者竞争互作并不清楚，而介导MC+与MC-微囊藻竞争互作的信号物质也待深究。通过培养基模拟—自然水体实验相互印证研究，本项目从蛋白质组、代谢组学的全新角度揭示不同氮磷水平下MC+、MC-藻细胞（单培、混培）应对木犀草素胁迫的响应机制，基于组学数据并运用色—质谱联用等技术进一步鉴定介导MC+、MC-微囊藻竞争互作的藻源信号物质，以全面深入阐释木犀草素对MC+、MC-微囊藻生长生理及两者竞争互作的调控效应和机理（包括是否影响了介导互作的信号物质的合成），为加强木犀草素控制微囊藻水华提供深层次理论依据和技术支撑。

Microcystis-dominated algal blooms (MDABs), coupled with microcystins (MCs)-production, severely threaten ecosystem-safety. It reached a consensus that MC-producing (MC+) Microcystis and MC-free (MC-) Microcystis always co-exist to form competitive interaction with each other in aquatic habitats, greatly affects MDABs toxicity and toxicity-dynamics. Our study found that plant-originated allelochemical—luteolin could inhibit the growth of MC+ Microcystis and its MC-production, and showed the potential implications for MDABs-remediation. However, how luteolin affects the growth and physiological characteristics of MC+ and MC- Microicystis, respectively, at different nitrogen and phosphorus levels was still unclear. Also, the signaling compounds mediating the competitive interaction between MC+ and MC- Microcystis deserved further elucidation. From proteomic—metabolomic views, this proposal aimed to provide novel understanding into growth, cellular and physiological responsive mechanisms for MC+ and MC- Microcystis exposing to luteolin at various nitrogen and phosphorus levels, through monoculture, co-culture and field samples studies. Based on omics data, this proposal applied chromatography-mass spectrometry technique to further identify Microcystis-originated signaling compound(s) mediating the competitive interaction between MC+ and MC- Microcystis. The findings would shed a deep interpretation on the influence mechanisms of luteolin on growth and physiological characteristics of MC+ and MC- Microcystis and on their competitive interaction (including luteolin-stress on the synthesis of Microcystis-originated signaling compounds), and provide further theory evidences and technical supports for luteolin-involved MDABs-management and -control.