Cav1.3（L-type ɑ1D）和PMCA2是分布于耳蜗的钙离子通道，对于维护毛细胞和内淋巴钙离子梯度稳定和机械电信号传导及听力至关重要。老年性聋是多因素作用的年龄相关听力损失，我们多年研究分别发现Cav1.3（L-type ɑ1D）和PMCA2在听觉中的重要作用，发现其与老年性聋发病明确相关。拟采用不同基因型小鼠（L-type ɑ1D+/-、PMCA2+/-L和L-type ɑ1D+/-/ PMCA2+/-）小鼠作为实验对象，建立动物模型，利用听觉生理、细胞培养、分子生物学、Ca2+微电极检测技术、免疫荧光电镜和激光扫描共聚焦显微镜实验、毛细胞分离培养技术和离子通道膜片钳技术等方法研究Cav1.3（L-type ɑ1D）和PMCA2在耳蜗中的功能和年龄相关性聋的变化，以期初步揭示耳蜗Cav1.3（L-type ɑ1D）和PMCA2在听觉机制及老年性聋中的致病作用。

Cav1.3 (L-type ɑ1D) and PMCA2 are calcium ion channels, which distributed in the cochlea, There are may to maintain cochlear hair cells and lymphatic calcium gradient within the stability and mechanical electrical signal conduction and hearing. Presbycusis is an age-related hearing loss of the role of various factors, the early research found Cav1.3 (L-type ɑ1D) and PMCA2 role are involved in auditory mechanisms espectively, them are also associated with the onset of age-related deafness. We Intends to adopt different genotype mice (L–type ɑ1D+/-；PMCA2+/-；L-type ɑ1D+/-/L-type ɑ1D+/-；PMCA2+/-/PMCA2+ /- and L-type ɑ1D+/-/PMCA2+/-) mice as experimental object, establish animal model of presbycusis using auditory physiology, cell culture, molecular biology, Calcium microelectrode detection technology, mmunofluorescence electron microscope and laser scanning confocal microscope, hairy cell culture techniques and the methods of ion channels patchclamp technique research Cav1.3 (L-type ɑ1D) and PMCA2 function and the change of the age-related deafness in the cochlea, in order to reveal preliminarily Cav1.3 (L-type ɑ1D) and PMCA2 in auditory mechanisms and the pathogenic role of presbycusis.