胶乳再生是橡胶树产胶细胞（乳管）的主要代谢活动，是橡胶树应答割胶胁迫的一种次生代谢，同时是橡胶产量形成的决定环节。转录调控在植物的胁迫应答、次生代谢中起着重要作用，而目前并不清楚乳管内的转录因子是如何介导胶乳再生调控的。本项目将在全基因组水平上系统鉴定乳管内表达的转录因子及其候选靶基因，利用不同产胶水平橡胶树品种和产量调控手段（乙烯刺激），分析候选转录因子及其靶基因的共表达与胶乳产量形成的相关性，结合相关胶乳代谢物测定，筛选鉴定控制胶乳再生的关键转录因子及其调控的功能基因，并通过酵母单杂、荧光定量PCR以及转基因等技术，验证转录因子与启动子的结合和转录激活活性，最终构建调控乳管代谢的主要转录因子调控网络。项目结果，有助于揭示乳管中转录因子参与胶乳再生调控的分子机制，充实和完善橡胶生物合成的调控理论，为橡胶树高产定向遗传改良提供理论指导。

Latex regeneration, the main metabolism occuring in laticifers of Hevea brasiliensis (para rubber tree), is a kind of secondary metabolism that responds to to the tapping stress, as well as a rate-limiting step for latex production. Transcriptional regulation plays a principal role in plant stress response and secondary metabolism. Hitherto, it remains unclear how the transcription factors (TF) are involved in regulating latex regeneratin. In this project, we will extensively screen the TFs and their candidate target genes in laticifers, and identify the key TFs and their target genes involved in latex regeneration. We will investigate the correlation between latex yield and TF-target gene coexpression as well as metabolic analysis, using the Hevea varieties with differing yielding and the ethylene-stimulation technique. The specific TF-target gene relationship will be determined by the analyses of qRT-PCR，yeast one hybrid and transgenic technology. Our ultimate target is to unravel the major TF-involved gene expression regulatory network in laticifers. The results of this project will help understand the molecular mechanisms underlying TF-involved latex regeneration in laticifiers, enrich the theory of rubber production, and provide theoretical guidance to yield-oriented genetic improvement in Hevea.