The Calcium Sensor Calcineurin B-Like Proteins -Calcineurin B-Like Interacting Protein Kinases Is Involved in Leaf Development and Stress Responses Related to Latex Flow in Hevea brasiliensis.
The Calcium Sensor Calcineurin B-Like Proteins -Calcineurin B-Like Interacting Protein Kinases Is Involved in Leaf Development and Stress Responses Related to Latex Flow in Hevea brasiliensis.
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钙传感器 CBL-CIPK 参与巴西橡胶树的叶片发育和与乳胶流相关的应激反应
DOI：
10.3389/fpls.2022.743506
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发表时间：
2022
影响因子：
5.6
通讯作者：
Tang C
中科院分区：
文献类型：
--
作者：
Xiao X;Mo C;Sui J;Lin X;Long X;Qin Y;Fang Y;Tang C
巴西橡胶树(Para橡胶树)是天然橡胶(顺式-1，4-聚异戊二烯，NR)的唯一商业来源，其胶乳流动使其成为唯一适合于植物胁迫反应研究的产品。钙调神经磷酸酶B-样相互作用蛋白激酶(CIPK)作为钙敏感蛋白，与类钙调神经磷酸酶(CBL)反应，在植物发育过程中的激素信号转导和对非生物胁迫的响应中发挥重要作用。然而，人们对它们在橡胶树上的功能知之甚少。本研究从橡胶树基因组中鉴定出12个CBL(HbCBL)基因和30个CIPK(HbCIPK)基因。结构和系统发育分析将这些CIPK分为5个组，CBL分为4个组，并定位到橡胶树18条染色体中的14条上。RNA-seq和qPCR分析表明，HbCBL和HbCIPK基因在橡胶树的7个组织中的表达存在差异，这7个组织是：乳胶、树皮、叶、根、种子、雌花和雄花。2个HbCBL和16个HbCIPK基因的表达在叶片发育过程中呈上升趋势。在乙烯产量刺激和割胶处理后，这两种做法都会引发胁迫，大多数胶乳表达的基因的表达水平都发生了显著变化。酵母双杂交试验表明，HbCBL和HbCIPK的多种组合相互作用，在胶乳或其他橡胶树组织中有大量的基因表达。然而，所有被研究的HbCBL-HbCIPK复合体都不能招募HbSOS1或AtSOS1在酵母细胞中形成功能性的耐盐SOS途径。综上所述，这些结果表明橡胶树CBL-CIPK网络在生长、发育和与胶乳生产相关的胁迫反应中作为几个不同信号通路的汇合点。
Latex flow in Hevea brasiliensis (the Para rubber tree), the sole commercial source of natural rubber (cis-1,4-polyisoprene, NR), renders it uniquely suited for the study of plant stress responses. Calcineurin B-like interacting protein kinases (CIPK) serving as calcium-sensor protein kinases react with calcineurin B-like proteins (CBL) to play crucial roles in hormone signaling transduction and response to abiotic stress in plant developmental processes. However, little is known about their functions in Hevea. In this study, a total of twelve CBL (HbCBL) and thirty CIPK (HbCIPK) genes were identified from the Hevea genome. Structure and phylogenetic analysis assigned these CIPKs to five groups and CBLs to four groups, and mapped onto fourteen of the eighteen Hevea chromosomes. RNA-seq and qPCR analysis showed that the expressions of HbCBL and HbCIPK genes varied in the seven Hevea tissues examined, i.e., latex (cytoplasm of rubber-producing laticifers), bark, leaf, root, seed, female flower, and male flower. The expressions of two HbCBL and sixteen HbCIPK genes showed upward trends during leaf development. Following ethylene yield stimulation and the latex tapping treatment, both practices invoking stress, the expression levels of most latex-expressed genes were significantly altered. Yeast two-hybrid test revealed interactions for multiple combinations of HbCBLs and HbCIPKs with substantial gene expression in latex or other Hevea tissues. However, all the HbCBL-HbCIPK complexes examined did not recruit HbSOS1 or AtSOS1 to form functional salt tolerance SOS pathway in yeast cells. Taken together, the results suggested a role of the Hevea CBL-CIPK network as a point of convergence for several different signaling pathways in growth, development, and stress responses in relation to latex production.
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