没有任何一项技术发明像表面增强拉曼散射(SERS)光谱般，在发现了半个世纪后仍然没有 得以广泛的实际应用，但围绕其应用的研究方兴未艾；原因就在于拉曼光谱反应了独特的物质 标识特征。表面增强拉曼散射旨在于增强拉曼散射微弱的散射截面，使其能够成为研究、应 用的实际光谱工具。由于历史原因，研究者人为地将SERS复杂的增强机理划分为物理的电磁因 素和化学的电荷转移因素。然而这种截然分割在理论及实验上并不能完全揭示拉曼增强统一的 内在本质，也无法使现有理论与多数实验及应用实现明确的融合。本项目旨在利用先进的针尖 增强拉曼散射，在实验中实现拉曼增强的变量分离，精细呈现拉曼增强各个因素的分离、弱耦 合和强耦合相互作用；并以此精密的实验为依据对拉曼计算理论进行修正，引入局域场效应的校正 。拉曼增强的实验及理论的彻底诠释，不仅能使定量SERS测量成为物质光谱检测的可靠依据； 也将使DNA拉曼测序成为可能。

Surface-enhanced Raman scattering (SERS) spectroscopy has not been applied broadly since its discovery in more than half contraries, but the research efforts have been active extensively. The reason lies in its unique material identification feature spectrum. SERS was intended to enhance the extremely weak Raman scattering cross section, so that it can become a practical tool for spectral application. Due to historical reasons, the researchers divided the complex mechanism into charge transfer as chemical enhancement factors and electromagnetic effect as the physical enhancement factors. However, the categorization did not reveal the inner nature of the Raman enhancement. It hardly integrates the existing theory and most experiments. This project aims to utilize advanced tip-enhanced Raman scattering, to achieve a separation of abovementioned enhancement variables, showing various effects of factors, such as the weak coupling and strong coupling between the two enhancement factors. The refined basis for experiments will be employed to correct current Raman theory of computation by considering the local field effect. By the interpretation of experiments and theory on SERS, not only will the project make a reliable basis for the quantitative measurement of SERS spectra, but SERS　based DNA sequencing would also become possible in precision medical applications.