Ph基因系统保证小麦“二倍化”遗传。ph-KL基因是不同于Ph1和Ph2基因的新基因，该基因自然存在于小麦地方品种，能够促进小麦-外源杂种的部分同源染色体配对，因此在创制小麦-外源易位染色体方面有重要价值。但到目前为止，还不清楚该基因在染色体上的位置及其连锁的分子标记。本项目旨在：（1）利用小麦-黑麦杂种单倍体群体，以高通量SNP标记初步定位ph-KL基因；（2）进一步，基于SSR标记以及A、D基因组和中国春基因组信息，开发出连锁更紧密、易于追踪检测ph-KL基因的PCR标记；（3）结合细胞学观察，验证利用PCR标记转移ph-KL基因的有效性。本研究工作为该基因在外源基因转移中的应用及进一步对其精细定位和遗传作用机制解析奠定基础。

The diploid-like inheritance of common wheat is ensured by the Ph gene system. The ph-like gene, ph-KL, existing in the natural population of common wheat landrace, is different from the genes Ph1 and Ph2 that prevent homoeologous chromosome pairing (HCP). In contrast, gene ph-KL promotes HCP in wheat-alien hybrids and hence provides the usefulness in developing wheat-alien translocation chromosomes. Until recently, however, we still unknown its’ location on chromosome and linked molecular markers. Our aims in this program are: (1) to preliminary map the ph-KL gene using haploid segregation populations derived from wide hybridization between wheat and rye with the help of high-thought single nucleotide polymorphism markers; (2) to further develop PCR markers that are more closely linked to ph-KL and more easily detected according to previously reported SSR markers, the A/D genome sequences of diploid donor species and the draft genome sequences of Chinese Spring; (3) to validate the efficiency of newly developed PCR markers in transferring ph-KL into other genetic background by combing cytological observation with marker analysis. These works will lay a foundation for the utilization of this gene in alien gene transferring and its further fine mapping and genetic mechanism elucidating.