部分同源染色体配对促进位点QPh.sicau-3A的精细定位和候选基因分析

Ph基因系统保障了小麦减数分裂中同源染色体的正常配对，操作Ph基因是诱导小麦-外源染色体易位的重要手段。中国小麦地方品种开县罗汉麦具有促进部分同源染色体配对的隐性ph基因。申请者将其主效位点QPh.sicau-3A定位在3A染色体长臂的696-725Mb区段。本项目拟精细定位QPh.sicau-3A，筛选候选基因，验证其功能。预期该研究结果，对深入解析减数分裂过程中，同源染色体和部分同源染色体的识别、染色体联会和重组的分子机理具有重要意义。

Ph gene system ensures that chromosome pairing exclusively occurs between homologous chromosomes during meiosis. Manipulating the system is an important method to induce wheat-alien translocation. Kaixian-luohanmai, Chinese wheat landrace, has recessive ph gene(s) promoting homoeologous chromosome pairing. In a previous study, we mapped such a major QTL, QPh.sicau-3A, to 696-725 Mb region of chromosome arm 3AL. In the present project, we will try to finely map QPh.sicau-3A, screen candidate genes, and to verify the functions. We expect that our works are helpful to further understand the molecular mechanisms of how homologous and homoeologous chromosomes recognition, synapses and recombination during meiosis in polyploid species.