头颈鳞癌（HNSCC）是头颈部癌症中最常见的类型之一，对中晚期患者无非常有效的治疗措施，探索其具体发生发展机制可为其寻找新的治疗靶点提供理论基础。本课题组前期通过全基因表达谱芯片检测△P63α基因在鳞癌细胞中敲减后基因表达谱的改变，筛选到一个重要的基因泛素结合酶E2S(UBE2S)；临床研究发现ΔNp63α在鳞癌组织中高表达，并与UBE2S的表达呈正相关；细胞实验发现ΔNp63α可调控UBE2S表达，并影响鳞癌细胞的生长周期；UBE2S的敲减同样影响鳞癌细胞的增殖、凋亡及周期。我们推测ΔNp63α通过促进UBE2S的表达促进头颈鳞癌的发生发展，并可能影响其预后。本项目拟将干预鳞癌细胞中ΔNp63α的表达，研究ΔNp63α调控UBE2S影响鳞癌细胞生物学功能的机制，并结合动物实验和临床样本检测探索ΔNp63α影响头颈鳞癌发生发展的分子机制，为其临床治疗寻找新的治疗靶点提供新思路。

Head and neck squamous cell carcinoma (HNSCC) is one of the most common types in head and neck cancers. There is no prominently effective diagnosis and treatment methods for the advanced cancer patients and the prognosis is poor. Our previous research identify a important gene UBE2S through the study of gene expression profiling of ΔNP63α knockdown HNSCC cells；Clinical research showed that ΔNp63α highly expressed in cancer tissues of HNSCC, and the expression level of ΔNp63α was positively related to UBE2S. The further cellular level studies revealed the regulation roles of ΔNp63α on UBE2S. The knockdown of UBE2S also affects the proliferation, apoptosis and cell cycle of HNSCC cells. Therefore, we speculate that ΔNp63α could inhibit the expression of UBE2S to promote the tumorigenesis and development of HNSCC, which might also lead to poor prognosis. This project aimed to study how ΔNp63α regulate and control UBE2S to influence the biological function of HNSCC cells, explore the molecular mechanisms that ΔNp63α regulate and control UBE2S to effect the occurrence, development and prognosis of HNSCC, and provide new idea and therapy target for HNSCC treatment in clinic.