羊痘病毒（CaPV）是毒力最强的动物痘病毒，其基因组呈线形，分中间的核心区和两侧可变区。KLP1位可变区，已证明其有明显的致病作用。但其生物学功能和致病的分子机理还不清楚。本研究拟以新疆南疆分离的山羊痘病毒aks株为研究对象，克隆表达KLP1蛋白，经分子筛，初步确定其分子的存在形式；提取病羊组织总RNA,构建其cDNA文库，用酵母双杂交的方法筛选KLP1的互作蛋白，并用ELISA、CO-IP、PULL-DOWM和哺乳动物双杂交的方法检测确证互作蛋白的真实性；采用RNA干扰的方法研究靶蛋白对羊痘病毒复制的影响，利用荧光共定位技术确定互作蛋白的作用部位及强度；构建KLP1基因分段缺失质粒，用细胞同源重组的方法筛选不同缺失的病毒株，研究KLP1主要致病的基因片段，并用生物软件分析，明确其生物学功能，推测其分子致病机理，为进一步了解Kelch蛋白、深入研究羊痘病毒、防治羊痘发生提供理论依据。

Capripoxvirus (CaPV) is an animal pox virus with strongest virulence and linear genome. It includes an core area in the middle parts and an variable area on both sides. KLP1 lies in variable area. it has been proven to have strong pathogenicty. But its bilogical function and pathogenic molecular mechanism are not clear. This study intends to take a goatpox virus of aks strain isolated from from xinjiang southern as the research object , cloning and expressing KLP1 protein,Preliminary determining its molecular form by using molecular sieve; extracting Total RNA of diseased sheep tissue and constructing cDNA library, screenning protein interactions of KLP1 by using yeast two hybrid method,and vaoidating its accuracy by using ELISA, CO - IP, a PULL - DOWM and mammalian two hybrid method .Studying the effect of target protein on replication of goatpox virus by using RNA interference method ,determing the positions and intensity of interacting protein using fluorescence co-localization technology, constructing stepnise deletion plasmied of KLP1 screenning virus strains with different deletion ,using homelogous recombination methed,exploring the main pathogenic fragments of KLP1 gene,Analysing and darifying its biological function using biologiical softwaer and speculating mokecular pathogenic mechanism which can providing theoretical basis for further nuderstanding the kelch protein,the studying of goatpox virus and preventing and trsating goatpox.