ABC转运蛋白介导的CdTe量子点外排与毒性机制研究

随着纳米技术的发展，越来越多的量子点被合成并应用于生物医学研究及人们的日常生活中，然而量子点潜在的细胞毒性限制了其应用的展开，同时也给生态环境及人体健康造成了巨大威胁。考虑到量子点毒性很大程度上受其自细胞排出过程的影响，本项目拟以CdTe量子点为研究对象，以肝肾细胞为模型，系统研究ABC转运蛋白（ATP Binding Cassette Transporters）介导量子点外排的现象、过程与调控机理，以及该过程对量子点毒性的影响。进一步的，根据理化性质及核受体等因素对量子点外排的调控作用，我们设计量子点开发与应用方案，并通过细胞实验验证该方案的实用性。本项目的研究有助于完善量子点的毒理学与代谢学理论，为其开发与毒性防治提供新的参考，同时也可促进其在生物医药领域的安全应用。

With the development of Nano-techniques, more and more quantum dots are developed and applied in biomedical investigation and human daily life. However, such application is limited by the potential toxicity of quantum dots, and they are causing a severe threat to the environment and human health. Since the toxicity of quantum dots are significantly affected by their excretion process, this project aims to use liver/kidney cells to investigate the involvement of ATP binding cassette transporters (ABC transporters) in the efflux and toxicity of CdTe quantum dots, as well as the modulation mechanism and details of this process. By studying the effects of physicochemical properties, nuclear receptors and other factors on the efflux rate of quantum dots, we would offer a new proposal for the design and application of quantum dots, which could be tested by cellular experiments. This study will help to complement the mechanism for the toxicity and metabolism of quantum dots , providing a new law for the development and detoxification of quantum dots, and promoting their applications in the field of biomedicines.