糖尿病性骨质疏松症(DOP)是特指糖尿病导致的骨质疏松，在我国逐渐被认识并重视。DOP是牙列缺损的重要病因，影响种植牙骨结合。干细胞是组织再生的基础，也是骨结合的基础。DOP可导致干细胞功能异常，但其原因与调控机理有待阐明。我们的研究表明DOP状态下骨髓间充质干细胞（BMSCs）成骨分化能力降低，Wnt通路发挥一定调控作用。此外，近期研究表明不同组织来源干细胞功能存在差异，我们发现人颌骨BMSCs成骨能力优于股骨BMSCs，此外，老年颌骨BMSCs骨再生能力明显高于中年股骨BMSCs。本项目拟在前期基础上全面比较正常和DOP条件下颌骨BMSCs的生物学特性变化，阐明调控颌骨BMSCs成骨分化的分子机制，在此基础上建立恢复DOP条件下颌骨BMSCs再生功能的方法。意义在于不仅开发颌骨来源的种子细胞，还揭示DOP对干细胞功能影响和组织再生的调控机制，为提高DOP患者种植牙成功率提供理论依据。

Diabetes-induced Osteoporosis (DOP) have been gradually recognized and attention in our country，which may lead to the occurrence of oral disease and affect the treatment. To improve the success ratio of dental implant for patients with tooth loss accompanied with diabetes is a difficulty but research hot topic in the dental implant field, and the key is to enhance the implant osteointegration. Stem cell is the key point of tissue regeneration. Recent studies suggested that the biological functions of stem cell are changes under DOP condition，in addition the key cytokines which affecting its biological function were not clear and the mechanism is still unknown. Our previous studies showed that：the proliferation and osteogenesis differentiation were reduce in BMSCs from DOP condition and the expression of miR-182 was increased in BMSCs; the Wnt signaling pathway play an important role in MSCs osteogenesis differentiation progress. Our previous studies have been successfully obtained MSCs from the alveolar bone marrow, and confirmed in situ mandibular alveolar bone formation ability of MSCs to be significantly stronger than the bone marrow MSCs. We will compare the biological features of BMSC and PDLSC from normal donors and DOP patients, and find out the important impact cytokines which affect the function of stem cells from DOP patients. Elucidate the molecular mechanism of stem cell functional abnormality caused by DOP defects and find the possible way that agents modifying the pathway could be of value to bone regeneration in DOP patients. This study may provide a theoretical basis for stem cell tissue engineering on dental implant applying in patients with tooth loss in combination with DOP.