驱动蛋白在动物精细胞核的形态建成中作用的分子机制悬而未决。中华绒螯蟹的成熟精核呈杯状，其染色质为非高度折叠型。KIFC1是一种C-末端驱动蛋白，我们的前期研究表明该蛋白在中华绒螯蟹精细胞核中的表达呈从中期到后期精细胞逐步增加的趋势，后期精细胞核中KIFC1大量分布。KIFC1入核是否参与精细胞核形态变化以及如何发挥作用迄今无人知晓。我们已发现kifc1的过表达和敲低均与细胞核的形态和结构变化有密切关系, 因此推测在中华绒螯蟹精核形成过程中, KIFC1蛋白可能通过与染色质DNA结合、并替换组蛋白以及与B型核纤层蛋白结合等途径参与精核的形态建成。本研究拟在培养生精细胞和在体水平上，用基因过表达和RNA干扰方法，探究KIFC1与组蛋白、核纤层蛋白及染色质之间的相互作用，旨在系统研究KIFC1在中华绒螯蟹非浓缩精核形成中的作用。本研究将首次证明kinesin-14家族具有染色体驱动蛋白的功能。

The molecular mechanism of kinesin involved in the spermatid morphogenesis in animals remains largely unknown. The nucleus of the spermatids in Eriocheir sinensis are cup-shaped and chromatins are not highly condensed. KIFC1 belongs to the C-terminal kinesin subfamily, our previous research have demonstrated that KIFC1 gradually accumulated in the nucleus of spermatids at middle stage and robustly expressed in the nucleus of spermatids at late stage. The relationships between the nuclear import and biological functions of KIFC1 and the spermatid morphogenesis remain unknown. We also have discovered that both overexpression and knockdown of kifc1 led to the abnomal in the shape and structure of nucleus, and then we hypothesize that KIFC1 may bind to the chromatin, involved in the exclusion of histones and interacts with lamin B to regulate the spermatid morphogenesis during the spermiogenesis of Eriocheir sinensis. This study plan to take advantage of the overexpression and knockdown assays to study the interactions of KIFC1, histones, lamin and chromatin in nucleus of cultured spermatogenic cells and in vivo. We aim to systematically study the biological functions of KIFC1 in the formation of uncondensed nucleus of spermatids of Eriocheir sinensis. This study may reveal the new functions of kinesin-14 subfamily as the chromokinesin.