精原细胞有丝分裂及其调控对于维持动物生殖能力至关重要，迄今精原细胞有丝分裂调控及其动力学机制悬而未决。精巢发育过程中Wnt和BMP信号分子可促进动物精原细胞分裂，但其转运动力未知。本项目组已获得中华绒螯蟹Wnt5a、Wnt6、BMP2、KIF3A/3B和KIFC1基因，我们拟研究该物种精原细胞有丝分裂中Wnt和BMP通路的调控及转运机制。本项目组已发现KIF3A通过介导β-catenin核质分布参与Wnt通路调控。我们的工作假设是驱动蛋白可能通过转运Wnt和BMP通路中信号分子促进精原细胞增殖。课题组拟在原代培养精原细胞和活体水平上，使用过表达、RNA干扰、蛋白互作、活细胞成像和CRISPR-Cas9基因敲除技术，研究中华绒螯蟹KIF3A/3B与KIFC1如何通过转运与调节Wnt和BMP信号分子促进精原细胞的分裂。本研究将首次证明精原细胞分裂过程中Wnt和BMP信号分子通路的动力学机制。

The mechanisms underlying Crustacean reproductive system are among the hot spots of current academia concerns. Spermatogonial mitosis and its regulation are vital for maintaining sufficient numbers of mature sperms and fertility. However, the regulation and the precise mechanism of spermatogonial mitosis remain unknown. Previous studies have shown that during animal testis development, Wnt and BMP signaling molecules can promote spermatagonial mitosis, whereas the kinetics are unknown. Our laboratory has obtained the full-length Eriocheir sinensis wnt5a, wnt6, bmp2, kif3a/3b and kifc1 genes, we plan to study the transportation and regulation of Wnt and BMP pathways during E. sinensis spermatogonial mitosis. We have obtained data that KIF3A may participate in Wnt pathways through regulating the distribution of β-catenin. We suppose that kinesins may participate in spermatagonial proliferation and maintaining cell numbers via transporting Wnt and BMP signal molecules. We will use Chinese mitten crab E. sinensis as our animal model, and utilize over-expression, RNA interference, protein interactions, live-cell imaging and CRISPR-Cas9 knockout techniques in this project. Our aim is to reveal that kinesin motors KIF3A/3B and KIFC1 may regulate spermatogonial mitosis via transporting Wnt and BMP signaling molecules. Our study will firstly reveal the kinetic mechanisms of Wnt and BMP signaling molecules during E. sinensis spermatagonial mitosis.