前列腺素类化合物作为局部激素通过激活其特定受体在排卵、受精以及胚胎发育等生殖过程中发挥重要作用，但尚不清楚前列腺素类化合物对子宫内膜上皮细胞增殖的影响及其作用机制。本项目以奶牛子宫内膜作为研究对象，采用体外细胞及组织培养、实时无标记细胞检测、膜片钳技术、流式细胞术、ELISA、荧光定量PCR、Western blot、免疫荧光染色等实验技术，研究PGE2和PGF2α对子宫内膜上皮细胞增殖的促进作用；研究PGE2和PGF2α对奶牛子宫内膜上皮细胞合成分泌VEGF、CTGF、TGF和IL-8等细胞组织生长相关因子的作用；分析PGE2和PGF2α是否通过子宫内膜上皮细胞PI3K/Akt信号转导通路的激活对细胞增殖进行调控。阐明PGE2和PGF2α促进子宫内膜上皮细胞增殖的机制，从而为临床上合理应用前列腺素类药物防治奶牛繁殖障碍疾病提供理论和实验依据。

Prostaglandins play important roles in female reproductive processes that include ovulation, fertilization, implantation, decidualization and placentation through their specific receptors. However, it is unclear that the influence of prostaglandins in endometrial cell proliferation and its mechanism of action. In this project, bovine endometrium is used as the main research object, which illuminates the effects of prostaglandin E2 and prostaglandin F2α on acceleration of the endometrial epithelial cells proliferation and growth factors including VEGF, CTGF, TGF and IL-8 secretion in bovine endometrial epithelial cells. Meanwhile, the regulatory roles of PI3K/Akt signaling in endometrial epithelial cell proliferation were analyzed by using the methods of cell and tissue culture in vitro, real-time cellular analysis, patch clamp recording technique, flow cytometry, ELISA, real-time quantitative PCR, Western blot and immunofluorescence staining. The results will illuminate the mechanism of prostaglandin E2 and prostaglandin F2α promoting the endometrial epithelial cells proliferation and provide theoretical and experimental basis for treatment of bovine reproductive system diseases using prostaglandins clinically.