在已获得壮骨止痛方防治绝经后骨质疏松有效部位和主要有效成分的基础上，本项目从骨免疫学角度推论其通过调节CD4+CD25+Tregs/Th17平衡实现抗骨质疏松的作用机制假说，综合利用去势雌鼠模型和雌激素受体敲除鼠病理模型验证假说，建立CD4+CD25+Tregs、Th17、OC的细胞培养体系及共培养体系，从细胞水平进一步验证假说，探寻其调节CD4+CD25+Tregs／Th17平衡的作用环节，最后，利用蛋白芯片筛选其调节细胞信号传导通路，并利用RT-PCR、Western blot技术验证蛋白芯片实验结果，然后根据信号传导实验结果反推其细胞因子或激素。本项目不仅阐明壮骨止痛方防治绝经后骨质疏松的骨免疫学机制，更深入、更精细地阐明壮骨止痛方多环节、多途径、多靶点的作用特点，而且能为临床更好应用壮骨止痛方提供指导，并为构建中西医结合骨免疫学提供宝贵实验资料，促进中西医结合骨免疫学的发展。

Zhuangguzhitong formula could prevent women from post-menopausal osteoporosis, but the mechanisms keep unveil. This subject proposed the hypothesis that Zhuangguzhitongfang could keep the balance between CD4+CD25+ regulatary T cells(CD4+CD25+Tregs) and Th17 in bone . Then four sub-hypothesis have been proposed to the mechanisms on keeping the balance of CD4+CD25+Tregs and Th17. OVX and ERKO were used to identify the hypothesis. Then co-culture of CD2+CD2+Tregs、T17、OB and OC will be found to study the inter-reaction between them. At last, the signalling path will be screened with the protein chip,and the RT-PCR and western blot will be used to identify the signalling path. Not only will the mechanisms be explained in the project, but also the direction to the clinic will be gotten, what`s more, the data to osteoimmunology of TCM will be gotten.