壮骨止痛方对绝经后骨质疏松症具有良好防治作用，抑制破骨细胞ER／RANK信号通路是壮骨止痛方抗绝经后骨质疏松症的重要作用机理，但尚不完全清楚其对破骨细胞ER／RANK信号通路的具体作用细节及其调控机理。本项目以破骨细胞ER／RANK／NFATc1信号通路为中心，提出壮骨止痛方可能从miRNA水平调节破骨细胞ER／RANK／NFATc1信号通路的研究假说。先从动物实验和细胞实验初步验证研究假说，并进一步应用antagomir和agomir技术从细胞和动物水平探讨壮骨止痛方调控破骨细胞ER／RANK／NFATc1信号通路的机理，同时通过检测对miRNA成熟过程相关基因的影响探讨壮骨止痛方调控miRNA的机理。本项目首次从miRNA探讨壮骨止痛方调控破骨细胞ER／RANK／NFATc1信号通路机理，不仅丰富和深化中医药抗绝经后骨质疏松症作用机理，而且为临床更好地应用壮骨止痛方提供指导。

Zhuanggu Zhitong decoction has showed satisfied effect on postmenopausal osteoporosis. Inhibiting ER / RANK signaling pathway in osteoclast is an important mechanism of Zhuanggu Zhitong decoction on postmenopausal osteoporosis, but it is not entirely clear on the osteoclast ER / RANK pathway details and regulation mechanisms. This project is based on the osteoclast ER / RANK / NFATc1 signaling pathway and puts forward research hypothesis that miRNAs maybe the targets regulated by Zhuanggu Zhitong decoction which regulate the ER/RANK/NFATc1 signaling pathway in osteoclasts. At first, animal experiments and cell experiments will be designed to verify the hypothesis,then antagomir and agomir technology will be further applied to explore the mechanisms of Zhuanggu Zhitong prescription regulation of osteoclast ER / RANK / NFATc1 signaling pathway in cell and animal level, meanwhile, the mechanismsthat Zhuanggu Zhitong prescription regulatesthe miRNA will be unveiled through detecting those gene expression regulating miRNA maturation. This project is the first time to investigate from the miRNA level Zhuanggu Zhitong prescription regulation of ER / RANK / NFATc1 signaling mechanism in osteoclasts from gene post-transcription and further clarify Zhuanggu Zhitong prescription regulation of osteoclast signal transduction mechanism, which will provide guidance for the clinical application of Zhuanggu Zhitong prescription better.